عنوان

Further characterization of tRNA(Trp) genes of Saccharomyces cerevisiae

Number

TLpq304057231

.Language of Text, Soundtrack etc

انگلیسی

Title Proper

Further characterization of tRNA(Trp) genes of Saccharomyces cerevisiae

General Material Designation

[Thesis]

First Statement of Responsibility

M. A. Riazi

Name of Publisher, Distributor, etc.

University of Alberta (Canada)

Date of Publication, Distribution, etc.

1992

Specific Material Designation and Extent of Item

118

Dissertation or thesis details and type of degree

M.Sc.

Body granting the degree

University of Alberta (Canada)

Text preceding or following the note

1992

Text of Note

Two goals were pursued in this work. First, attempts were made to construct an intronless ochre suppressor tRNATrp gene. The ability of the construct to function as an ochre suppressor was studied in Saccharomyces cerevisiae in vivo. The in vitro-constructed intronless tRNATrp gene suppressed the tested ochre mutations. This result supported models emphasizing the importance of the intron-anticodon stem loop binding in the splicing of pre-tRNA transcripts. These interactions are supposedly disrupted after a CCA to TTA change in the anticodon of the putative suppressor tRNATrp gene. An intronless gene would not have this problem since the need for splicing is abolished. The other interesting aspect of this work was the effect of an extrachromosomal factor (usd\Psi\sp{+}usd). The ability of the constructed tRNATrp gene to suppress one of the tested ochre mutations (lys1-1) was significantly increased in the presence of this factor, and reduced when this factor was eliminated. In the second part of this work the 5 and 3 flanking regions of the six tRNATrp genes in Saccharomyces cerevisiae were studied. (Abstract shortened by UMI.)

Biological sciences

Genetics

M. A. Riazi

Electronic name

p

[Thesis]

276903

a

Y