عنوان

DR5-AS uzun kodlamayan RNA'sının hücre proliferasyonu üzerine olan etkisinin araştırılması

Number

TLpq2522841702

.Language of Text, Soundtrack etc

انگلیسی

Title Proper

DR5-AS uzun kodlamayan RNA'sının hücre proliferasyonu üzerine olan etkisinin araştırılması

General Material Designation

[Thesis]

First Statement of Responsibility

Gürer, Dilek Cansu

Subsequent Statement of Responsibility

Akgül, Bünyamin

Name of Publisher, Distributor, etc.

Izmir Institute of Technology (Turkey)

Date of Publication, Distribution, etc.

2020

Specific Material Designation and Extent of Item

63

Dissertation or thesis details and type of degree

Master's

Body granting the degree

Izmir Institute of Technology (Turkey)

Text preceding or following the note

2020

Text of Note

Cell proliferation is the process of increasing cell number in a multicellular organism. In literature, there are numerous proteins and non-coding RNAs reported as regulators of cell proliferation, yet, many of others are waiting to be explored. Unravelling the mechanism behind the regulation of cell proliferation is crucial to develop new strategies for fighting numerous diseases such as cancer, immune diseases, or neurodegenerative diseases. Long non-coding RNAs (lncRNAs) are known to regulate various cellular processes. To determine which ones are related to cell proliferation and apoptosis in HeLa cells, a transcriptomics study was performed under cisplatin, doxorubicin, TNF-α and Anti-Fas treatments. DR5-AS is a novel lncRNA transcript selected from this transcriptomics study as a promising regulatory lncRNA candidate due to its overlap with DR5 protein-coding gene which is known to regulate apoptosis and proliferation. Several phenotypic characterization methods were performed to understand the function of DR5-AS lncRNA. These studies showed that DR5-AS knockdown causes a significant decrease in cell proliferation, an alteration in the normal HeLa cell morphology, a shift through S and G2/M phases in cell cycle profile, and significant accumulation of cells in the metaphase phase. A second transcriptomics study was performed with DR5-AS knockdown HeLa cells to uncover which pathways are responsible for these changes. The results suggest that DR5-AS lncRNA regulates expression of numerous key proteins in cell cycle regulation. This observation was confirmed by several qPCR experiments. In conclusion, this study provides the first evidence that DR5-AS lncRNA modulates cell cycle and proliferation in HeLa cells.

Cancer

Genetic engineering

Genetics

Molecular biology

Akgül, Bünyamin

Gürer, Dilek Cansu

Electronic name

p

[Thesis]

276903

a

Y