عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
NATIONAL BIBLIOGRAPHY NUMBER
Number
TL58204
LANGUAGE OF THE ITEM
.Language of Text, Soundtrack etc
انگلیسی
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
İnsanda apoptozu düzenleyen uzun kodlamayan RNA'ların moleküler karakterizasyonu
General Material Designation
[Thesis]
First Statement of Responsibility
Sweef, Osama
Subsequent Statement of Responsibility
Akgül, Bünyamin
.PUBLICATION, DISTRIBUTION, ETC
Name of Publisher, Distributor, etc.
Izmir Institute of Technology (Turkey)
Date of Publication, Distribution, etc.
2019
GENERAL NOTES
Text of Note
149 p.
DISSERTATION (THESIS) NOTE
Dissertation or thesis details and type of degree
Ph.D.
Body granting the degree
Izmir Institute of Technology (Turkey)
Text preceding or following the note
2019
SUMMARY OR ABSTRACT
Text of Note
Apoptosis is an evolutionarily form of programmed cell death for development and tissue homeostasis. Apoptosis is regulated by protein-coding genes and plays an important role in a wide range of biological processes. We aimed to identify and characterize differentially expressed lncRNAs in apoptosis. HeLa cells were used as a model system to identify the lncRNAs. The total RNAs was subjected to deep sequencing by next-generation sequencing. OmicsBOX Bioinformatics tools were used for differential expression analysis of lncRNAs that are apoptosis-induced. Gene set enrichment analysis (GSEA) was used to profile the miRNAs targeting lncRNAs. Cytoscape software was used to reconstruct lncRNA-miRNA targeting networks. RT-qPCR was used to validate miRNAs and their targets of lncRNAs and it was found that the overexpression of miR-519d-3p causes downregulation of lncRNAs RAB22A-202, PARD3-211, and AC027237.1-210. Also, the overexpression of miR-124-3p down-regulates the expression level of APEX2-202 and CD59-209. GTF2A1-AS, TNFRSF10B-AS, and CAMTA1-DT were detected in the nucleus and have no poly (A) tail and they belong to TATA-less promoter genes. TNFRSF10B-AS has a coding probability of 0.99 and alignment to High-scoring Segment Pair (HSP) clarifies one hit to Q9UBN6 protein. ChIRP clarifies that TNFRSF10B-AS binds to a protein (25 kDa). miR-519d-3p and miR-124-3p interact with lncRNA targets by miRNA-mediated lncRNA degradation pattern under apoptosis conditions. TNFRSF10B-AS has a putative regulatory function in the nucleus during apoptosis via binding specifically to the ribonucleoprotein partner.
UNCONTROLLED SUBJECT TERMS
Subject Term
Apoptosis
Subject Term
Genetics
Subject Term
Molecular biology
PERSONAL NAME - PRIMARY RESPONSIBILITY
Sweef, Osama
PERSONAL NAME - SECONDARY RESPONSIBILITY
Akgül, Bünyamin
CORPORATE BODY NAME - SECONDARY RESPONSIBILITY
Izmir Institute of Technology (Turkey)
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب p
[Thesis]
276903
a
Y
