Comparison of Led Fluorescent Microscopy, Zeihl-Neelsen and GeneXpert Assay for Direct Detection of Mycobacterium tuberculosis Complex in Sputum in Jos Plateau State, Nigeria
General Material Designation
[Thesis]
First Statement of Responsibility
Aguokoli, Augustina Ifunanya
Subsequent Statement of Responsibility
Adebayo, O.
.PUBLICATION, DISTRIBUTION, ETC
Name of Publisher, Distributor, etc.
Kwara State University (Nigeria)
Date of Publication, Distribution, etc.
2019
GENERAL NOTES
Text of Note
60 p.
DISSERTATION (THESIS) NOTE
Dissertation or thesis details and type of degree
M.S.
Body granting the degree
Kwara State University (Nigeria)
Text preceding or following the note
2019
SUMMARY OR ABSTRACT
Text of Note
This study compared the effectiveness and sensitivity of three different diagnostic methods of identification of pulmonary tuberculosis. These were Led fluorescent microscopy, Zeihl-Neelsen staining technique and GeneXpert assay for detection of the bacterium in sputum specimen. A cross sectional study was conducted among 435 patient suspected to have pulmonary tuberculosis at the Bingham Teaching Hospital Jos Plateau State Nigeria. The patients sputum were collected over three months period, 196 (45%) were males while 239 (55%) were females. For all specimens' collected Z-N detected 54 (12.4%) positive cases, FM detected 46 (10.6%) positive cases while the GeneXpert detected 57(13.1%) positive cases. All patients were subjected to test for human immunodeficiency virus (HIV), only 55 (28.1%) of males were HIV positive while 94 (39.3%) females were HIV positive. Although the identification identification techniques used were found to be specific, the result showed that GeneXpert was most sensitive and accurate than the other two. Consequently health care professionals may be encouraged to use the GeneXpert more since it very fast, more realable and it can also detects the resistance of rifampicin in the sample alongside with detection of the bacterium.