عنوان

Protocols for nucleic acid analysis by nonradioactive probes.

(Number (ISBN

1280832703

(Number (ISBN

1588294307

(Number (ISBN

1597452297

(Number (ISBN

6610832706

(Number (ISBN

9781280832703

(Number (ISBN

9781588294302

(Number (ISBN

9781597452298

(Number (ISBN

9786610832705

Number

b795326

Title Proper

Protocols for nucleic acid analysis by nonradioactive probes.

General Material Designation

[Book]

Edition Statement

2nd ed. /

Statement of Responsibility Relating to Edition

edited by Elena Hilario, John Mackay.

Place of Publication, Distribution, etc.

Totowa, N.J. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©2007.

Specific Material Designation and Extent of Item

1 online resource (x, 321) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology,

Volume Designation

353

ISSN of Series

1064-3745 ;

Text of Note

Includes bibliographical references and index.

Text of Note

Genomic DNA isolation from different biological materials / Duckchul Park -- Extraction of plant RNA / Elspeth MacRae -- Overview of hybridization and detection techniques / Elena Hilario -- Checkerboard DNA-DNA hybridization technology : using digoxigenin detection / Lisa S. Gellen, Glenn M. Wall-Manning, and Chris H. Sissons -- Nonradioactive northern and southern analyses from plant samples / Christoph Peterhaensel, Dagmar Weier, and Thomas Lahaye -- Screening a BAC library with nonradioactive overlapping oligonucleotide (overgo) probes / Elena Hilario, Tiffany F. Bennell, and Erik Rikkerink -- Direct in-gel hybridization of DNA with digoxigenin-labeled probes / Saeed A. Khan and Mohamed S. Nawaz.

Text of Note

In situ hybridization of termite microbes / Shigeharu Moriya [and others] -- RNA electrophoretic mobility shift assay using a fluorescent DNA sequencer / Yukinori Eguchi -- Comparative quantitation of mRNA expression in the central nervous system using fluorescence in situ hybridization / Darren J. Day, Eli M. Mrkusich, and John H. Miller -- Visualization of gene expression by fluorescent multiplex reverse transcriptase-PCR amplification / María Rosa Ponce [and others] -- Fluorescence in situ hybridization for the identification of environmental microbes / Annelie Pernthaler and Jakob Pernthaler -- Introduction to kinetic ("real-time") PCR / John Mackay -- Validation of short interfering RNA knockdowns by quantitative real-time PCR / Sukru Tuzmen, Jeff Kiefer, and Spyro Mousses.

Text of Note

Real-time quantitative PCR as an alternative to southern blot or fluorescence in situ hybridization for detection of gene-copy number changes / Jasmien Hoebeeck, Frank Speleman, and Jo Vandesompele -- Design and work-up of a new molecular diagnostic assay based on real-time PCR / Harald H. Kessler -- Real-time PCR fluorescent chemistries / John Mackay and Olfert Landt -- Microarrays : an overview / Norman H. Lee and Alexander I. Saeed -- Oligonucleotide microarrays for the study of coastal microbial communities / Gaspar Taroncher-Oldenburg and Bess B. Ward.

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Text of Note

Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Title

Protocols for nucleic acid analysis by nonradioactive probes.

International Standard Book Number

9781588294302

Nucleic acid probes.

Nucleic acids-- Analysis.

DNA-- analysis.

DNA Probes.

Molecular Probe Techniques.

Nucleic Acid Amplification Techniques.

DNA-- Analysis.

DNA probes.

Molecular Probe Techniques.

Nucleic Acid Amplification Techniques.

Nucleic acid probes.

Nucleic acid probes.

Nucleic acids-- Analysis.

Nucleic acids-- Analysis.

SCIENCE-- Life Sciences-- Genetics & Genomics.

PSB.

SCI-- 029000

Number

Edition

Class number

Book number

Class number

Class number

Class number

Book Number

Book Number

System Code

Hilario, Elena,1966-

Mackay, John,1969-

Date of Transaction

20201207082322.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y