عنوان
پدید آورنده
موضوع
رده
QP519.9.A35 H54 1999
QP519
.
9
.
A35
H54
1999
کتابخانه
محل استقرار
INTERNATIONAL STANDARD BOOK NUMBER
(Number (ISBN
0191565660
(Number (ISBN
058548399X
(Number (ISBN
1280375477
(Number (ISBN
661037547X
(Number (ISBN
9780191565663
(Number (ISBN
9780585483993
(Number (ISBN
9781280375477
(Number (ISBN
9786610375479
Erroneous ISBN
0199636486 (pbk.)
Erroneous ISBN
0199636494
NATIONAL BIBLIOGRAPHY NUMBER
Number
b779188
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
High resolution chromatography :
General Material Designation
[Book]
Other Title Information
a practical approach /
First Statement of Responsibility
edited by Paul Millner.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
New York :
Name of Publisher, Distributor, etc.
Oxford University Press,
Date of Publication, Distribution, etc.
©1999.
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
1 online resource (xx, 311 pages) :
Other Physical Details
illustrations.
SERIES
Series Title
Practical approach series ;
Volume Designation
204
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index.
CONTENTS NOTE
Text of Note
Intro; Contents; List of contributors; Abbreviations; Section A. Techniques and equipment; 1. Separation by charge, size, and hydrophobicity; 1. Introduction; 2. Choice of separation method; 3. Choice of hardware; 4. Packing columns; 5. Running the column; References; 2. Practice and benefits of microcolumn HPLC; 1. Introduction; 2. Practice of microcolumn HPLC; 3. Conclusion; References; 3. Detection devices; 1. Introduction; 2. The classification of detectors; 3. Detecting devices for high efficiency columns; 4. Light absorption detectors; 5. Fluorescence detectors; 6. Electronic detectors
Text of Note
4. Use of lectins: practical aspects5. Lectin affinity chromatography of oligosaccharides, glycosylasparagines, and glycopeptides; References; 6. Nucleotide- and dye-ligand chromatography; 1. Introduction; 2. Nucleotide-ligand chromatography; 3. Dye-ligand chromatography; References; 7. Synthetic peptides as affinity ligands; 1. Introduction; 2. Coupling peptides via amine groups directly to the matrix; 3. Coupling peptides via sulfydryl groups; 4. Coupling peptides and introducing spacer arms using carbodiimides; 5. Chromatography on peptide affinity gels; References
Text of Note
7. Equipment and operationReferences; 10. Strategies and methods for purification of Ca[sup(2+)]-binding proteins; 1. Introduction; 2. Purification of the annexin Ca[sup(2+)]-binding proteins; 3. The heparin column; 4. The Chelex-100 competitive Ca[sup(2+)]-binding assay; References; 11. Purification of DNA-binding proteins; 1. General procedure for DNA-binding proteins; 2. Preparation of nuclear extracts; 3. Heparin-Sepharose; 4. DNA affinity chromatography for purification of sequence specific DNA-binding proteins; References; Appendix; Index; A; B; C; D; E; F; G; H; I; L; M; N; O; P; Q; R
Text of Note
7. Light scattering detectorsReferences; 4. Capillary electrophoresis of peptides and proteins; 1. Introduction; 2. The capillary; 3. Buffer; 4. Sample preparation; 5. Modes used in CE; 6. CE detection strategies; 7. Applications of CE to peptide and protein research; 8. CE of peptides and proteins: future prospects; References; Section B. Selected affinity approaches; 5. Lectins as affinity probes; 1. Properties and uses of lectins-a historical perspective; 2. The types of oligosaccharides found in glycoproteins; 3. Carbohydrate recognition and lectin specificity
Text of Note
8. Drugs and inhibitors as affinity ligands1. Introduction; 2. Choice of ligand; 3. Choice of matrix; 4. Choice of spacer arm; 5. Coupling method; 6. Determination of ligand coupling efficiency; 7. Affinity chromatography; 8. Method of elution; 9. Affinity chromatography with biotinylated ligands; 10. Regeneration of the affinity matrix; Acknowledgements; References; 9. Immunoaffinity chromatography; 1. Introduction; 2. Antibody selection; 3. Applications of immunoaffinity separations; 4. Matrices; 5. Activation and immobilization; 6. Determining coupling efficiency
0
8
8
8
8
SUMMARY OR ABSTRACT
Text of Note
The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification.
OTHER EDITION IN ANOTHER MEDIUM
Title
High resolution chromatography.
International Standard Book Number
9780199636495
TOPICAL NAME USED AS SUBJECT
Affinity chromatography.
Biomolecules-- Purification.
Proteins-- Purification.
Biomolécules-- Purification.
Chromatographie d'affinité
Protéines-- Purification.
Affinitätschromatographie
Affinitätschromatographie.
Affiniteitschromatografie.
Affinity chromatography.
Biomoleculen.
Biomolecules-- Purification.
Biomolécules-- Purification.
Chromatographie
Chromatographie d'affinité.
Chromatographie en phase liquide à hautes performances.
Chromatographie.
HPLC
HPLC.
HPLC.
Kapillarelektrophorese
Kapillarelektrophorese.
Methode
Methode.
Protéines-- Purification.
Proteins-- Purification.
SCIENCE-- Chemistry-- Analytic.
(SUBJECT CATEGORY (Provisional
X300
X500
SCI-- 013010
DEWEY DECIMAL CLASSIFICATION
Number
547/
.
75046
Edition
21
LIBRARY OF CONGRESS CLASSIFICATION
Class number
QP519
.
9
.
A35
Book number
H54
1999
OTHER CLASS NUMBERS
Class number
35
.
29
Class number
CHE
228f
Class number
CHE
232f
Class number
JF
30
Class number
VG
7200
Class number
WC
3480
System Code
bcl
System Code
stub
System Code
stub
System Code
blsrissc
System Code
rvk
System Code
rvk
PERSONAL NAME - ALTERNATIVE RESPONSIBILITY
Millner, Paul.
ORIGINATING SOURCE
Date of Transaction
20201206182148.0
Cataloguing Rules (Descriptive Conventions))
pn
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب [Book]
Y
