Includes bibliographical references (pages 419-424) and index.
CONTENTS NOTE
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DNA structure -- Genes and chromosomes -- DNA replication in bacteria -- DNA replication in eukaryotes -- RNA structure -- Transcription in prokaryotes -- Operons -- Transcription in eukaryotes: an overview -- Transcription of protein-coding genes in eukaryotes -- Regulation of transcription by RNA Pol II-- Processing of eukaryotic pre-mRNA -- Ribosomal RNA -- Transfer RNA.
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Introduction to enzymes -- Thermodynamics -- Enzyme kinetics -- Enzyme inhibition -- Regulation of enzyme activity -- The immune system -- Antibodies: an overview -- Antibody synthesis -- Antibodies as tools -- Membrane lipids -- Membrane proteins and carbohydrate -- Transport of small molecules -- Transport of macromolecules -- Signal transduction -- Nerve function.
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Prokaryote cell structure -- Eukaryote cell structure -- Cytoskeleton and molecular motors -- Bioimaging -- Cellular fractionation -- Amino acids -- Acids and bases -- Protein structure -- Myoglobin and hemoglobin -- Collagen -- Protein purification -- Electrophoresis of proteins -- Protein sequencing and peptide synthesis.
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Structures and roles of fatty acids -- Fatty acid breakdown -- Fatty acid synthesis -- Triacylglycerols -- Cholesterol -- Lipoproteins -- Citric acid cycle -- Electron transportand oxidative phosphorylation -- Photosynthesis -- Nitrogen fixation and assimilation -- Amino acid metabolism -- The urea cycle -- Hemes and chlorophylls.
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The genetic code -- Translation in prokaryotes -- Translation in eukaryotes -- Protein targeting -- Protein glycosylation -- The DNA revolution -- Restriction enzymes -- Nucleic acid hybridization -- DNA cloning -- DNA sequencing -- Polymerase chain reaction -- Monosaccharides and disaccharides -- Polysaccharides and oligosaccharides -- Glycolysis -- Gluconeogenesis -- Pentose phosphate pathway -- Glycogen metabolism -- Control of glycogen metabolism.
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SUMMARY OR ABSTRACT
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This volume presents an update, with changes in the content and organisation that reflect advances in the subject. New and expanded topics include cytoskeleton, molecular motors, bioimaging, biomembranes, cell signalling, protein structure, and enzyme regulation.