عنوان

Trinucleotide repeat protocols /

(Number (ISBN

1280360011

(Number (ISBN

1588292436

(Number (ISBN

1592598048

(Number (ISBN

6610360014

(Number (ISBN

9781280360015

(Number (ISBN

9781588292438

(Number (ISBN

9781592598045

(Number (ISBN

9786610360017

Number

b777058

Title Proper

Trinucleotide repeat protocols /

General Material Designation

[Book]

First Statement of Responsibility

edited by Yoshinori Kohwi.

Place of Publication, Distribution, etc.

Totowa, N.J. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©2004.

Specific Material Designation and Extent of Item

1 online resource (xii, 342 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology ;

Volume Designation

277

Text of Note

Includes bibliographical references and index.

Text of Note

Mouse models of triplet repeat diseases / Gillian P. Bates and David G. Hay -- Analysis of triplet repeat replication by two-dimensional gel electrophoresis / Maria M. Krasilnikova and Sergei M. Mirkin -- Genetic analysis for triplet repeat instability in yeast / Michael J. Dixon, Saumitri Bhattacharyya, and Robert S. Lahue -- Detection and isolation of trinucleotide repeat expansions using the RED method / Qiu-Ping Yuan and Martin Schalling -- Analysis of unstable triplet repeats using small pool polymerase chain reaction / Mário Gomes-Pereira, Sanjay I. Bidichandani, and Darren G. Monckton -- Real-time RT-PCR for CTG repeat-containing genes / Maria Eriksson -- Antibodies against Huntingtin: production and screening of monoclonals and single-chain recombinant forms / Ali Khoshnan [and others] -- Using antibodies to analyze polyglutamine stretches / Elizabeth Brooks [and others] -- Solubilization of aggregates formed by expanded polyglutamine tract expression in cultured cells / Noriko Hazeki and Ichiro Kanazawa -- Caenorhabditis elegans as a model system for triplet repeat diseases / Cindy Voisine and Anne C. Hart -- Monitoring aggregate formation in organotypic slice cultures from transgenic mice / Donna L. Smith and Gillian P. Bates -- The CGG repeat and the FMR1 gene / Violetta Stoyanova and Ben A. Oostra -- Analysis of CTG repeats using DM1 model mice / Cédric Savouret, Claudine Junien, and Genevieve Gourdon -- Lentiviral-mediated gene transfer to model triplet repeat disorders / Etienne Régulier ... [et at.] -- Mouse tissue culture models of unstable triplet repeats / Mário Gomes-Pereira and Darren G. Monckton -- Neurotransmitter receptor analysis in transgenic mouse models / Caroline L. Benn, Laurie A. Farrell, and Jang-ho J. Cha -- Chromatin immunoprecipitation technique for study of transcriptional dysregulation in intact mouse brain / Melissa W. Braveman [and others] -- Techniques for thick-section golgi impregnation of formalin-fixed brain tissue / Tracie L. Moss and William O. Whetsell, Jr -- Assessment of impaired proteasomal function in a cellular model of polyglutamine diseases / Nihar Ranjan Jana and Nobuyuki Nukina -- Assessment of in vitro and in vivo mitochondrial function in Friedreich's Ataxia and Huntington's Disease / Anthony Schapira and Raffaele Lodi -- Triplet repeats and DNA repair: germ cell and somatic cell instability in transgenic mice / Irina V. Kovtun, Craig Spiro, and Cynthia T. McMurray -- Oxidative damage in Huntington's Disease / José Segovia and Francisca Pérez-Severiano.

0

Text of Note

The discovery that trinucleotide repeats significantly influence the age of onset and severity of a variety of hereditary neurological disorders has opened the door to a deeper understanding of the disease mechanisms involved, as well as to a more productive search for novel therapeutic interventions. In Trinucleotide Repeat Protocols, established leaders in trinucleotide repeat disease describe in step-by-step detail their best techniques for studying trinucleotide pathology at the molecular level. The protocols cover a variety of targets, ranging from DNA and RNA to proteins and whole animals, and focus not only on causal genes, but also on their consequent products, such as transcription factors, neurotransmitter receptors, proteasomes, and mitochondria/oxidation damage. Experimental systems employed include E. coli, yeast, C. elegans, mouse, and generally take a clinical point of view. The authors utilize a wide range of techniques, including gel electrophoresis, quantitative RT-PCR, immunological analysis, antibody usage and its applications, receptor assays using radioisotope handling, gene delivery by virus, brain cell and organotypic cultures, gender dependency, and neuron structure analysis. Each protocol follows the successful Methods in Molecular Biology™ series format, offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Trinucleotide Repeat Protocols offers neuroscientists powerful tools to elucidate both normal brain function and the mechanisms of hereditary neurological disease, as well as to develop the next generation of therapies for neuronal genetic diseases.

Source for Acquisition/Subscription Address

00012511

Stock Number

1588292436

Title

Trinucleotide repeat protocols.

International Standard Book Number

1592598048

Trinucleotide repeats, Laboratory manuals.

Trinucleotide Repeats.

HEALTH & FITNESS-- Diseases-- Nervous System (incl. Brain)

MEDICAL-- Neurology.

Trinucleotide repeats.

HEA-- 039110

MED-- 056000

PSF.

Number

Edition

Class number

Book number

Class number

Class number

Book Number

Book Number

Kohwi, Yoshinori.

Date of Transaction

20201203101144.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y