عنوان

Directed evolution library creation :

(Number (ISBN

1280843233

(Number (ISBN

1588292851

(Number (ISBN

159259395X

(Number (ISBN

6610843236

(Number (ISBN

9781280843235

(Number (ISBN

9781588292858

(Number (ISBN

9781592593958

(Number (ISBN

9786610843237

Erroneous ISBN

9781617374715

Number

b768765

Title Proper

Directed evolution library creation :

General Material Designation

[Book]

Other Title Information

methods and protocols /

First Statement of Responsibility

edited by Frances H. Arnold and George Georgiou.

Place of Publication, Distribution, etc.

Totowa, N.J. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©2003.

Specific Material Designation and Extent of Item

1 online resource (x, 224 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology ;

Volume Designation

v. 231

Text of Note

Includes bibliographical references and index.

Text of Note

Cover -- Preface -- Contents -- Contributors -- MUTAGENESIS AND RECOMBINATION METHODS -- 1 Generating Mutant Libraries Using Error-Prone PCR -- 2 Preparing Libraries in Escherichia coli -- 3 Preparing Libraries in Saccharomyces cerevisiae -- 4 Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP) -- 5 Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis -- 6 Production of Randomly Mutated Plasmid Libraries Using Mutator Strains -- 7 Evolution of Microorganisms Using Mutator Plasmids -- 8 Random Insertion and Deletion Mutagenesis -- 9 Random Oligonucleotide Mutagenesis -- 10 Saturation Mutagenesis -- 11 DNA Shuffling -- 12 Family Shuffling with Single-Stranded DNA -- 13 In Vitro DNA Recombination by Random Priming -- 14 Staggered Extension Process (StEP) In Vitro Recombination -- 15 RACHITT -- 16 The Creation of ITCHY Hybrid Protein Libraries -- 17 Preparation of SCRATCHY Hybrid Protein Libraries -- 18 Sequence Homology-Independent Protein Recombination (SHIPREC) -- 19 Producing Chimeric Genes by CLERY -- ANALYSIS OF LIBRARY DIVERSITY -- 20 Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization -- 21 Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays -- 22 Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays -- Index -- Last Page.

0

Text of Note

Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Protocols, seasoned practitioners from many leading laboratories share their best experimental protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Taken together, Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications.

Title

Directed evolution library creation.

International Standard Book Number

9781588292858

Combinatorial chemistry, Laboratory manuals.

Gene libraries, Laboratory manuals.

Genetic engineering, Laboratory manuals.

Genetic recombination, Laboratory manuals.

Microbial mutation, Laboratory manuals.

Molecular evolution, Laboratory manuals.

Combinatorial Chemistry Techniques.

Gene Library.

Banques de gènes, Manuels de laboratoire.

Chimie combinatoire, Manuels de laboratoire.

Évolution moléculaire, Manuels de laboratoire.

Génie génétique, Manuels de laboratoire.

Micro-organismes-- Mutation, Manuels de laboratoire.

Recombinaison génétique, Manuels de laboratoire.

Biologia molecular.

Combinatorial chemistry.

Engenharia genética.

Gene libraries.

Genetic engineering.

Genetic recombination.

Genetica.

Genetische manipulatie.

Microbial mutation.

Moleculaire biologie.

Molecular evolution.

Mutagênese.

Recombinação genética.

SCIENCE-- Life Sciences-- Molecular Biology.

Wetenschappelijke technieken.

PSF.

SCI-- 049000

Number

Edition

Class number

Book number

Class number

Class number

Book Number

Book Number

Arnold, Frances Hamilton.

Georgiou, George.

Date of Transaction

20201206143132.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y