عنوان

Microscopy, immunohistochemistry, and antigen retrieval methods :

(Number (ISBN

0306467704

(Number (ISBN

0306475995

(Number (ISBN

9780306467707

(Number (ISBN

9780306475993

Erroneous ISBN

1280200871

Erroneous ISBN

6610200874

Erroneous ISBN

9781280200878

Erroneous ISBN

9786610200870

Number

b767527

Title Proper

Microscopy, immunohistochemistry, and antigen retrieval methods :

General Material Designation

[Book]

Other Title Information

for light and electron microscopy /

First Statement of Responsibility

M.A. Hayat.

Place of Publication, Distribution, etc.

New York :

Name of Publisher, Distributor, etc.

Kluwer Academic/Plenum Publishers,

Date of Publication, Distribution, etc.

©2002.

Specific Material Designation and Extent of Item

1 online resource (xviii, 355 pages) :

Other Physical Details

color illustrations

Text of Note

Includes bibliographical references (pages 305-350) and index.

Text of Note

Cover -- Preface -- Table of Contents -- Chapter 1. Introduction -- Cytogenetic Evaluation of Tumors -- Genetic Instability of Tumors -- Tumor Heterogeneity -- Antitumor Vaccines -- Molecular Genetics -- cDNA Microarray Technology -- Angiogenesis -- Future of Immunohistopathology -- Preparation of Buffers -- Chapter 2. Antigens and Antibodies -- Antigens -- Antibodies -- Recombinant Antibodies -- Anticancer Monoclonal Antibodies -- Antibody Cross-Reactivity -- Polyreactive Antibodies -- Commercial Sources of Antibodies -- Chapter 3. Fixation and Embedding -- Formaldehyde -- Effect of Heating on Fixation with Glutaraldehyde -- Microwave Heat ... Assisted Fixation with Osmium Tetroxide -- Role of Microwave Heating in Enzyme Cytochemistry -- Cryopreservation in the Presence of Microwave Heating -- Paraffin Embedding -- Chapter 4. Factors Affecting Antigen Retrieval -- Fixation -- Denaturation -- Heating -- pH -- Molarity -- Antigen Retrieval Fluids -- Antibody Penetration -- Antibody Dilution -- Storage of Paraffin-Embedded Tissues -- Storage of Tissue Slides -- Signal Amplification -- Chapter 5. Problems in Antigen Retrieval -- Lack of Immunostaining -- Background Staining -- Problem of Endogenous Biotin -- Mirror Image Complementary Antibodies -- Fixation of Frozen Tissues -- Hot Spots (Areas) in Microwave Oven -- Problem of Antigen Retrieval Standardization -- Test Battery -- Intraobserver and Interobserver Variation in Diagnosis -- Quantitation of Immunostaining -- Autostainers -- Volume-Corrected Mitotic Index -- The Gleason Grading System -- Universal Antigen Retrieval Method? -- Calibration of Microwave Oven -- Chapter 6. Antigen Retrieval -- Possible Mechanisms of Antigen Retrieval -- Effect of Endogenous Calcium on Antigen Masking -- Use of Ethylenediaminetetraacetic Acid (EDTA) for Antigen Retrieval -- Antigen Retrieval with Heat Treatment -- Advantages of Heating -- Heating Methods -- Wet Autoclave Method -- Ultrasound Treatment -- Nonheating Methods -- Comparison of Antigen Retrieval Methods: A Summary -- Chapter 7. Antigen Retrieval on Resin Sections -- Role of Fixative and Embedding Resin in Antigen Retrieval -- Immunostaining of Thin Resin Sections -- Antigen Retrieval on Sections of Modified Epoxy Resin -- Effect of Heating -- Antigen Retrieval on Thin Resin Sections Using Autoclaving -- Rapid Staining of Thin Resin Sections in Microwave Oven -- Microwave Heat ... Assisted Rapid Processing of Tissues for Electron Microscopy -- Microwave Heat ... Assisted Immunolabeling of Resin-Embedded Sections -- Microwave Heat ... Assisted Immunogold Methods -- Chapter 8. General Methods of Antigen Retrieval -- General Procedure for Antigen Retrieval Using Microwave Heating -- Antigen Retrieval in Archival Tissues -- Antigen Retrieval Using a Conventional Oven -- Hot Plate ... Assisted Antigen Retrieval -- Hot Plate ... Assisted Grading of Vulvar Intraepithelial Neoplasia -- Water Bath Heat ... Assisted Antigen Retrieval -- Microwave Heat ... Assisted Evaluation of Global DNA Hypomethylation -- Microwave Heat ... Assisted Enhanced.

0

Text of Note

Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.

Title

Microscopy, immunohistochemistry, and antigen retrieval methods.

International Standard Book Number

0306467704

Antigens.

Electron microscopy.

Immunohistochemistry.

Microscopy.

Antigens-- isolation & purification.

Immunohistochemistry.

Microscopy.

Antigens.

Electron microscopy.

Immunohistochemistry.

MEDICAL-- Histology.

Microscopy.

MED-- 110000

Number

Edition

Class number

Book number

Class number

Book Number

Hayat, M. A.,1936-

Date of Transaction

20201206125841.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y