عنوان

Fluorescence in situ hybridization (FISH) :

(Number (ISBN

1607617897

(Number (ISBN

9781607617891

Erroneous ISBN

1607617889

Erroneous ISBN

9781607617884

Number

b760802

Title Proper

Fluorescence in situ hybridization (FISH) :

General Material Designation

[Book]

Other Title Information

protocols and applications /

First Statement of Responsibility

edited by Joanna M. Bridger, Emanuela V. Volpi.

Place of Publication, Distribution, etc.

New York, NY :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©2010.

Specific Material Designation and Extent of Item

1 online resource (xiv, 451 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology,

Series Title

Springer protocols

Volume Designation

659

ISSN of Series

1940-6029 ;

Text of Note

Includes bibliographical references and index.

Text of Note

Fluorescence in situ Hybridization (FISH), Basic principles and methodology -- Fluorescence in situ hybridization on DNA halo preparations and extended chromatin fibres -- Detection of nascent RNA transcripts by fluorescence in situ hybridization -- Fluorescence in situ hybridization analysis of formalin fixed paraffin embedded tissues, including tissue microarrays -- Fluorescence in situ Hybridization (FISH) on tissue cryosections -- Multiplex fluorescence in situ ybridization (M-FISH) -- Optical mapping of protein-DNA complexes on chromatin fibers -- 3D-FISH on cultured cells combined with immunostaining -- The Comet-FISH assay for the analysis of DNA damage and repair -- Direct in situ hybridization with oligonucleotide functionalized quantum dot probes -- LNA-FISH for detection of MicroRNAs in frozen sections -- Chromosome orientation fluorescence in situ hybridization or strand-specific FISH -- Combinatorial oligo FISH: directed labeling of specific genome domains in differentially fixed cell material and live cells -- Simultaneous visualization of FISH signals and bromo-deoxyuridine Incorporation by formamide-free DNA denaturation -- CryoFISH: fluorescence in situ hybridization on ultrathin cryosections -- Characterization of chromosomal rearrangements using multicolor-banding (MCB/m-band) -- Visualizing nucleic acids in living cells by fluorescence in vivo hybridization -- Quality control in FISH as part of a laboratory's quality management system -- FlashFISH: "same day" prenatal diagnosis of common chromosomal aneuploidies -- FISH for pre-implantation genetic diagnosis -- PNA-FISH on human sperm -- POD-FISH: a new technique for parental origin determination based on copy number variation polymorphism -- Sequence-based high resolution chromosomal comparative genomic hybridization (CGH) -- ImmunoFISH on isolated nuclei from paraffin-embedded biopsy material -- Fluorescence in situ hybridization on 3D cultures of tumor cells -- Simultaneous ultrasensitive subpopulation staining/hybridization in situ (SUSHI) in HIV-1 disease mnitoring -- Detection of prokaryotic cells with fluorescence in situ hybridization -- FISH as a tool to investigate chromosome behavior in budding yeast -- FISH on chromosomes derived from the snail model organism biomphalaria glabrata -- Fluorescence in situ hybridization with bacterial artificial chromosomes (BACs) to mitotic heterochromatin of drosophila -- Three-dimensional fluorescence in situ hybridization in mouse embryos using repetitive probe sequences -- Fluorescence in situ hybridization (FISH) for genomic investigations in rat -- Fluorescence in situ hybridization on early porcine embryos -- FISH on 3D preserved bovine and murine preimplantation embryos.

0

Text of Note

The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.

Source for Acquisition/Subscription Address

Springer

Stock Number

978-1-60761-788-4

Title

Fluorescence in situ hybridization (FISH).

Fluorescence in situ hybridization.

Situ Hybridization, Fluorescence

Biology.

Fluorescence in situ hybridization.

Genetics.

Health & Biological Sciences.

Medicine.

Pathology.

PSB

SCI007000

Number

Edition

Class number

Book number

Class number

Book Number

Bridger, Joanna M.

Volpi, Emanuela V.

Date of Transaction

20201204202913.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y