عنوان

LC-MS/MS in proteomics :

(Number (ISBN

1607617803

(Number (ISBN

9781607617808

Erroneous ISBN

160761779X

Erroneous ISBN

9781607617792

Number

b753472

Title Proper

LC-MS/MS in proteomics :

General Material Designation

[Book]

Other Title Information

methods and applications /

First Statement of Responsibility

edited by Pedro R. Cutillas, John F. Timms.

Place of Publication, Distribution, etc.

New York, NY :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©2010.

Specific Material Designation and Extent of Item

1 online resource (x, 357 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology,

Series Title

Springer protocols

Volume Designation

658

ISSN of Series

1064-3745 ;

Text of Note

Includes bibliographical references and index.

Text of Note

Approaches and applications of quantitative LC-MS for proteomics and activitomics / Pedro R. Cutillas and John F. Timms -- Overview of quantitative LC-MS techniques for proteomics and activitomics / John F. Timms and Pedro R. Cutillas -- Instrumentation for LC-MS/MS in proteomics / Robert Chalkley -- Bioinformatics for LC-MS/MS-based proteomics / Richard J. Jacob -- Analysis of post-translational modifications by LC-MS/MS / Hannah Johnson and Claire E. Eyers -- In-depth analysis of protein phosphorylation by multidimensional ion exchange chromatography and mass spectrometry / Maria P. Alcolea and Pedro R. Cutillas -- Mapping of phosphorylation sites by LC-MS/MS / Bertran Gerrits and Bernd Bodenmiller -- Analysis of carbohydrates on proteins by offline normal-phase liquid chromatography MALDI-TOF-MS/MS / Theodora Tryfona and Elaine Stephens -- Selected reaction monitoring applied to quantitative proteomics / Reiko Kiyonami and Bruno Domon -- Basic design of MRM assays for peptide quantification / Andrew James and Claus Jorgensen -- Proteome-wide quantitation by SILAC / Kristoffer T.G. Rigbolt and Blagoy Blagoev -- Quantification of proteins by iTRAQ / Richard D. Unwin -- Quantification of proteins by label-free LC-MS/MS / Yishai Levin and Sabine Bahn -- Protocol for quantitative proteomics of cellular membranes and membrane rafts / Andrew J. Thompson and Ritchie Williamson -- Organelle proteomics by label-free and SILAC-based protein correlation profiling / Joern Dengjel, Lis Jakobsen, and Jens S. Andersen -- Mapping protein-protein interactions by quantitative proteomics / Joern Dengjel, Irina Kratchmarova, and Blagoy Blagoev -- Analysis of serum proteins by LC-MS/MS / Sarah Tonack, John P. Neoptolemos, and Eithne Costello -- Urinary proteome profiling using 2D-DIGE and LC-MS/MS / Mark E. Weeks -- Analysis of peptides in biological fluids by LC-MS/MS / Pedro R. Cutillas -- Quantification of protein kinase activities by LC-MS / Maria P. Alcolea and Pedro R. Cutillas -- A protocol for top-down proteomics using HPLC and ETD/PTR-MS / Sarah R. Hart.

0

Text of Note

With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.

Source for Acquisition/Subscription Address

Springer

Stock Number

978-1-60761-779-2

Title

LC-MS/MS in proteomics.

International Standard Book Number

9781607617792

Parallel Title

Liquid chromatography with tandem mass spectrometry in proteomics

Liquid chromatography.

Proteomics.

Tandem mass spectrometry.

Chromatography, Liquid-- methods.

Computational Biology-- methods.

Proteomics-- methods.

Tandem Mass Spectrometry-- methods.

Animal Biochemistry.

Health & Biological Sciences.

Human Anatomy & Physiology.

Liquid chromatography.

Proteomics.

Tandem mass spectrometry.

PSBC

SCI007000

Number

Edition

Class number

Book number

Class number

Book Number

Cutillas, Pedro R.

Timms, John F., (John Francis)

Date of Transaction

20201204130128.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y