عنوان

Human retrovirus protocols :

(Number (ISBN

1280359188

(Number (ISBN

1588294951

(Number (ISBN

1592599079

(Number (ISBN

6610359180

(Number (ISBN

9781280359187

(Number (ISBN

9781588294951

(Number (ISBN

9781592599073

(Number (ISBN

9786610359189

Number

b739855

Title Proper

Human retrovirus protocols :

General Material Designation

[Book]

Other Title Information

virology and molecular biology /

First Statement of Responsibility

edited by Tuofu Zhu.

Place of Publication, Distribution, etc.

Totowa, NJ :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

2005.

Specific Material Designation and Extent of Item

1 online resource (xiv, 494 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology ;

Volume Designation

v. 304

Text of Note

Includes bibliographical references and index.

Text of Note

Enhanced culture assay for detection and quantitation of latently infected, resting cd4+ t-cells carrying replication-competent virus in HIV-1-infected individuals / J.D. Siliciano and R.F. Siliciano -- Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals / H. Schuitemaker and N.A. Kootstra -- Isolation of human immunodeficiency virus type 1 from peripheral blood monocytes / P.R. Gorry [and others] -- Isolation, propagation, and HIV-1 infection of monocyte-derived macrophages and recovery of virus from brain and cerebrospinal fluid / S. Gorantla, M. Che and H.E. Gendelman -- Isolation and HIV-1 infection of primary human microglia from fetal and adult tissue / K. Borgmann, H.E. Gendelman and A. Ghorpade -- Isolation of human immunodeficiency virus type 1 from semen and vaginal fluids / A.A. Kiessling -- Isolation and quantification of HIV from lymph nodes / C. Tamalet -- Isolation of human immunodeficiency virus type 2 biological clones from peripheral blood lymphocytes / H. Blaak -- Isolation and confirmation of human t-cell leukemia virus type 2 from peripheral blood mononuclear cells / M.D. Lairmore and A. Montgomery -- Isolation of foamy viruses from peripheral blood lymphocytes / J. Tobaly-Tapiero, P. Bittoun and A. Saib -- Alu-ltr real-time nested PCR assay for quantifying integrated HIV-1 DNA / A. Brussel, O. Delelis and P. Sonigo -- Quantification of hfv-integrated DNA in human cells by alu-ltr real-time PCR / O. Delelis, A. Brussel and P. Sonigo -- Detection of HIV-1 provirus and rna by in situ amplification / A. Nicol and G.J. Nuovo -- Detection of htlv-1 gene on cytologic smear slides / K. Kashima, T. Daa and S. Yokoyama -- Detection of HIV-2 by PCR / F. Gao -- Quantitation of HIV-1 viral rna in blood plasma and genital secretions / S.A. Fiscus -- Quantification of proviral DNA load of human immunodeficiency virus type 2 subtypes a and b using real-time PCR / M. Gueudin, F. Damond and F. Simon -- Plasma rna viral load in HIV-1 group o infection by real-time PCR / M. Gueudin and F. Simon -- A new combined HIV p24 antigen and anti-HIV-1/2/o screening assay / S. Polywka [and others] -- A new automated fourth-generation HIV screening assay with sensitive antigen detection module and high specificity / B. Weber -- Detection of antiviral antibodies using enzyme-linked immunosorbent assay / I.G. Winkler -- Plaque-reduction assays for human and simian immunodeficiency virus neutralization / A. Nordqvist and E.M. Fenyo -- Detection of drug-resistant HIV-1 strains / A.A. Kiessling, S.J. Eyre and B.D. Desmarais -- Determination of cell tropism of HIV-1 / N.A. Kootstra and H. Schuitemaker -- Determination of co-receptor usage of HIV-1 / H. Schuitemaker and N.A. Kootstra -- Quantitative evaluation of HIV and SIV co-receptor use with ghost(3) cell assay / D. Vodros and E.M. Fenyo -- Phenotypic characterization of blood monocytes from HIV-infected individuals / P.J. Ellery and S.M. Crowe -- Methods to determine HIV-1 ex vivo fitness / A. Abraha [and others] -- A yeast recombination-based cloning system to produce chimeric HIV-1 viruses and express HIV-1 genes / D.M. Moore [and others] -- PCRs amplification, cloning, and construction of HIV-1 infectious molecular clones from virtually full-length HIV-1 genomes / P.K. Ehrenberg and N.L. Michael -- Amplification and cloning of near full-length HIV-2 genomes / F. Gao -- Growth and manipulation of a human t-cell leukemia virus type 2 full-length molecular clone / M. Anderson and P.L. Green -- Construction and analysis of genomic, full-length infectious foamy virus DNA clones / R. Wirtz and M. Lochelt -- Molecular characterization of proteolytic processing of the gag proteins of human spumaretrovirus / K.I. Pfrepper and R.M. Flugel -- Assessing the relative efficacy of antiretroviral activity of different drugs on macrophages / S. Aquaro and C.F. Perno -- Gene expression profiling of HIV-1 infection using cdna microarrays / A.B. van 't Wout -- Determination of DC-sign and DC-signr repeat region variations / H. Liu and T. Zhu.

0

Text of Note

This book is a cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources.; There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.

Source for Acquisition/Subscription Address

Springer

Stock Number

978-1-58829-495-1

Title

Human retrovirus protocols.

International Standard Book Number

1592599079

Retroviruses, Laboratory manuals.

HIV-- isolation & purification.

Molecular Biology-- methods.

Retroviridae-- isolation & purification.

Rétrovirus, Manuels de laboratoire.

HEALTH & FITNESS-- Diseases-- Contagious.

MEDICAL-- Infectious Diseases.

Retroviruses.

HEA-- 039040

MED-- 022090

PSGL

Number

Edition

Class number

Book number

Class number

Class number

Book Number

Book Number

Zhu, Tuofu.

Date of Transaction

20201204005009.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y