عنوان

Cell-based assays for high-throughput screening :

(Number (ISBN

1603275444

(Number (ISBN

1603275452

(Number (ISBN

9781603275446

(Number (ISBN

9781603275453

Number

b730633

Title Proper

Cell-based assays for high-throughput screening :

General Material Designation

[Book]

Other Title Information

methods and protocols /

First Statement of Responsibility

edited by Paul A. Clemons, Nicola J. Tolliday, and Bridget K. Wagner.

Place of Publication, Distribution, etc.

New York :

Name of Publisher, Distributor, etc.

Humana,

Date of Publication, Distribution, etc.

©2009.

Specific Material Designation and Extent of Item

1 online resource (x, 218 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology,

Series Title

Springer protocols

Volume Designation

v. 486

ISSN of Series

1064-3745 ;

Text of Note

Includes bibliographical references and index.

Text of Note

Introduction: Cell-based assays for high-throughput screening / W.F. An and N.J. Tolliday -- High-throughput screening of model bacteria / S. Zlitni, J.E. Blanchard and E.D. Brown -- Cell-based assays to probe the ERK MAP kinase pathway in endothelial cells / M.R. Wyler [and others] -- Large-scale small-molecule screen using zebrafish embryos / C.C. Hong -- Whole-animal high-throughput screens: The C. elegans model / E.J. O'Rourke, A.L. Conery and T.I. Moy -- Whole-organism screening: Plants / A. Agee and D. Carter -- Fluorescence-based assays / W.F. An -- Reporter gene assays / A.M.F. Liu [and others] -- Screening for chemical inhibitors of heterologous proteins expressed in yeast using a simple growth-restoration assay / A.D. Balgi and M. Roberge -- Assay for isolation of inhibitors of her2-kinase expression / G. Chiosis and A.B. Keeton -- High-content screening: Flow cytometry analysis / B.S. Edwards [and others] -- High-throughput real-time PCR for detection of gene-expression levels / B.K. Wagner and Z. Arany -- Interpretation of uniform-well readouts / S. Josiah -- Extracting rich information from images / A.E. Carpenter.

0

Text of Note

As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.

Source for Acquisition/Subscription Address

Springer

Stock Number

978-1-60327-544-6

Title

Cell-based assays for high-throughput screening.

International Standard Book Number

9781603275446

Biological assay.

High throughput screening (Drug development)

Biological Assay.

Drug Discovery.

Drug Evaluation, Preclinical.

Biochemistry.

Biological assay.

Biology - General.

Biology.

Chemistry.

Dosage biologique-- Cahiers de laboratoire.

Essais biologiques.

Évaluation préclinique médicament-- Cahiers de laboratoire.

Health & Biological Sciences.

High throughput screening

High throughput screening (Drug development)

Pharmacie-- Recherche.

Pharmacy, Therapeutics, & Pharmacology.

Physical Sciences & Mathematics.

Untersuchungsmethode

Zelle.

PSB

SCI007000

Number

Edition

Class number

Book number

Class number

Class number

Book Number

Book Number

Clemons, Paul A.

Tolliday, Nicola J.

Wagner, Bridget K.

Date of Transaction

20201203133746.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y