عنوان

CDNA libraries :

(Number (ISBN

1617790648

(Number (ISBN

1617790656

(Number (ISBN

9781617790645

(Number (ISBN

9781617790652

Number

b730496

Title Proper

CDNA libraries :

General Material Designation

[Book]

Other Title Information

methods and applications /

First Statement of Responsibility

edited by Chaofu Lu, John Browse and James G. Wallis.

Place of Publication, Distribution, etc.

New York, NY :

Name of Publisher, Distributor, etc.

Humana,

Date of Publication, Distribution, etc.

©2011.

Specific Material Designation and Extent of Item

1 online resource (xi, 275 pages) :

Other Physical Details

illustrations (some color)

Series Title

Methods in molecular biology,

Series Title

Springer protocols

Volume Designation

729

ISSN of Series

1064-3745 ;

Text of Note

Includes bibliographical references and index.

Text of Note

Isolation of fungal infection structures from plant tissue by flow cytometry for cell-specific transcriptome analysis / H. Takahara, E. Endl and R. O'Connell -- Preparation of a high-quality cDNA library from a single-cell quantity of mRNA using chum-RNA / H. Nojima and T. Tougan -- Construction of a full-length cDNA library from castor endosperm for high-throughput functional screening / C. Lu, J.G. Wallis and J. Browse -- Full-length transcriptome analysis using a bias-free cDNA library prepared with the vector-capping method / S. Kato, M. Oshikawa and K. Ohtoko -- Construction of improved yeast two-hybrid libraries / R.H. Maier, C.J. Maier and K. Onder -- Normalization of full-length-enriched cDNA / E.A. Bogdanova [and others] -- Bioinformatic methods for finding differentially expressed genes in cDNA libraries, applied to the identification of tumour vascular targets / J.M. Herbert [and others] -- Enzymatic production of RNAi libraries from cDNAs and high-throughput selection of effective shRNA expression constructs / K. Sugao and K. Hirose -- Construction of small RNA CDNA libraries for high-throughput sequencing / C. Lu and V. Shedge -- Focusing mutations within random libraries to distinct areas: Protein domain library generation by overlap extension / A. Gratz and J. Jose -- Generation of families of construct variants using golden gate shuffling / C. Engler and S. Marillonnet -- Application of full-length cDNA resources to gain-of-function technology for characterization of plant gene function / Y. Kondou [and others] -- Construction of yeast surface-displayed cDNA libraries / S. Bidlingmaier and B. Liu -- Identification of protein/target molecule interactions using yeast surface-displayed cDNA libraries / S. Bidlingmaier and B. Liu -- SNP discovery by transcriptome pyrosequencing / W.B. Barbazuk and P.S. Schnable -- RNA-seq analysis of gene expression and alternative splicing by double-random priming strategy / M.T. Lovci [and others] -- Generation of a large catalog of unique transcripts for whole-genome expression analysis in nonmodel species / D. Bellin, A. Ferrarini and M. Delledonne.

0

Text of Note

The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years. In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries. The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology.

Source for Acquisition/Subscription Address

Springer

Stock Number

978-1-61779-064-5

Title

CDNA libraries.

International Standard Book Number

9781617790645

Antisense DNA.

DNA, Complementary.

Animal Biochemistry.

Antisense DNA.

Health & Biological Sciences.

Human Anatomy & Physiology.

MED107000

MFN

Number

Edition

Class number

Class number

Class number

Book Number

Browse, John.

Lu, Chaofu.

Wallis, James G.

Date of Transaction

20201203132511.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y