DICTYOSTELIUM DISCOIDEUM: MOLECULAR APPROACHES TO CELL BIOLOGY.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
Burlington :
Name of Publisher, Distributor, etc.
Elsevier Science,
Date of Publication, Distribution, etc.
1987.
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
1 online resource (533 pages).
SERIES
Series Title
Methods in Cell Biology ;
Volume Designation
v. 28
GENERAL NOTES
Text of Note
Chapter 10. Ultrastructural Localization of Cytoskeletal Proteins in Dictyostelium Amoebae.
CONTENTS NOTE
Text of Note
Front Cover -- Dictyostelium discoideum: Molecular Approaches to Cell Biology -- Copyright Page -- CONTENTS -- Preface -- PART I: GENERAL PRINCIPLES -- Chapter 1. Introductory Remarks and Some Biochemical Considerations -- I. Introductory Remarks -- ll. Some Biochemical Considerations -- lll. Concluding Remarks -- References -- Chapter 2. Cultivation and Synchronous Morphogenesis of Dictyostelium under Controlled Experimental Conditions -- l. Introduction -- ll. Cell Cultivation -- lll. Strain Maintenance -- IV. Strain Preservation -- V. Conditions for Synchronous Development.
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I. Introduction -- ll. Quantitation of Endocytotic Uptake -- lll. Adhesion Assay -- IV. Aggregation Assay -- V. Isolation of Mutants with Altered Cohesive Properties -- VI. Characterization of Mutants -- VII. Conclusions and Discussion -- References -- Chapter 7. Defining the Intracellular Localization Pathways followed by Lysosomal Enzymes in Dictyostelium discoideum -- I. Introduction -- ll. Pulse-Chase Labeling Analyses of Lysosomal Enzymes -- lll. Subcellular Fractionation -- IV. Synthesis in Vitro of Lysosomal Enzymes.
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IV. Mapping and Sequencing of Dictyostelium Genes -- V. Analysis of Transcripts -- VI. DNA-Mediated Transformation -- VII. Appendix -- References -- PART ll: VESICULAR TRAFFIC IN CELLS -- Chapter 5 . Purification and Characterization of Dictyostelium discoideum Plasma Membranes -- I. Introduction -- ll. Membrane Purification Procedures -- lll. Comparison of Plasma Membranes Obtained by Different Methods -- IV. Vesiculation and Sealing of Plasma Membranes -- V. Choosing a Membrane Isolation Procedure -- References -- Chapter 6 . Endocytosis and Recognition Mechanisms in Dictyostelium discoideum.
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V. Isolation and Characterization of Mutants Altered in the Modification, Processing, and Secretion of Lysosomal Enzymes -- VI. Summary and Future Perspectives -- References -- Chapter 8. The Axenic Mutations and Endocytosis in Dictyostelium -- l. Introduction -- ll. Methods -- lll. Discussion -- References -- PART lll: CELL MOTILITY -- Chapter 9. Introduction of Exogenous Molecules into the Cytoplasm of Dictyostelium discoideum Amoebae by Controlled Sonication -- I. Introduction -- ll. Sonication Loading -- lll. Applications of Sonication Loading -- References.
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VI. Appendix: Some Useful Media and Solutions -- References -- Chapter 3. Genetic Tools for Dictyostelium discoideum -- l. Introduction -- ll. Mutagenesis -- lll. Selections -- IV. Screening Techniques -- V. Mutant Analysis -- Vl. Genetic Exchange -- VII. Mitotic Crossing Over -- VIII. Mapping Cloned Sequences -- IX. DNA Transformation -- X . Discussion -- XI. Sample Genetic Study -- References -- Chapter 4. Molecular Biology in Dictyostelium: Tools and Applications -- I. Introduction -- ll. Nucleic Acid Preparations -- lll. Recombinant DNA Libraries.
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SUMMARY OR ABSTRACT
Text of Note
METHODS IN CELL BIOLOGY, VOLUME 28: DICTYOSTELIUM DISCOIDEUM: MOLECULAR APPROACHES TO CELL BIOLOGY.
OTHER EDITION IN ANOTHER MEDIUM
Title
METHODS IN CELL BIOLOGY, VOLUME 28: DICTYOSTELIUM DISCOIDEUM: MOLECULAR APPROACHES TO CELL BIOLOGY.