عنوان

Free radical and antioxidant protocols /

(Number (ISBN

058523566X

(Number (ISBN

0896034720

(Number (ISBN

1280835982

(Number (ISBN

1592592546

(Number (ISBN

6610835985

(Number (ISBN

9780585235660

(Number (ISBN

9780896034723

(Number (ISBN

9781280835988

(Number (ISBN

9781592592548

(Number (ISBN

9786610835980

Number

b712987

Title Proper

Free radical and antioxidant protocols /

General Material Designation

[Book]

First Statement of Responsibility

edited by Donald Armstrong.

Place of Publication, Distribution, etc.

Totowa, N.J. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©1998.

Specific Material Designation and Extent of Item

1 online resource (xvii, 455 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology ;

Volume Designation

v. 108

Text of Note

Includes bibliographical references and index.

Text of Note

Oxygen consumption methods. Xanthine oxidase and lipoxygenase / I. Stadler -- Spin trapping and electron paramagnetic resonance spectroscopy / D.J. Brackett [and others] -- In vivo detection of free radicals in real time by low-frequency electron paramagnetic resonance spectroscopy / G.M. Rosen, S. Pou and H.J. Halpern -- Single photon counting / Y. Mizuguchi -- Measurement of reactive oxygen species in whole blood and mononuclear cells using chemiluminescence / K. Thusu, E. Abdel-Rahman and P. Dandona -- Assay of phospholipid hydroperoxides by chemiluminescence-based high-performance liquid chromatography / Y. Yamamoto, Y. Kambayashi and T. Ueda -- Sensitive and specific fluorescent probing of oxidative stress in different classes of membrane phospholipids in live cells using metabolically integrated CIS-parinaric acid / V.E. Kagan [and others] -- Aromatic hydroxylation. Salicylic acid as a probe for measuring hydroxyl radical production / A. Ghiselli -- Simple assay for the level of total lipid peroxides in serum or plasma / K. Yagi -- Simple procedure for specific assay of lipid hydroperoxides in serum or plasma / K. Yagi -- Antibodies against malondialdehyde-modified proteins. Induction and elisa measurement of specific antibodies / Y. Chancerelle, J. Mathieu and J.F. Kergonou -- Oxidized ldl and lp(a). Preparation, modification, and analysis / J. Galle and C. Wanner -- Oxidized and unoxidized fatty acyl esters / O. Sergent and J. Cillard -- Synthesis of lipid and cholesterol hydroperoxide standards / D. Armstrong and R.W. Browne -- Separation of hydroxy and hydroperoxy polyunsaturated fatty acids by high-pressure liquid chromatography / R.W. Browne and D. Armstrong -- Products of creatinine with hydroxyl radical as a useful marker of oxidative stress in vivo / K. Aoyagi [and others] -- Quantitative analysis of peptide and protein changes in ischemic hippocampal tissue by HPLC / T.M. Wengenack, J.R. Slemmon and J.M. Ordy -- Electrochemical detection of 8-hydroxy-2-deoxyguanosine levels in cellular DNA / T.M. Nicotera and S. Bardin -- Nitric oxide synthase / A. Aljada and P. Dandona -- Quantitation of heme oxygenase (ho-1) copies in human tissues by competitive RT/PCR / N.G. Abraham -- A fluorescent-based assay for measurement of phospholipase a2 activity. A facile assay for cell sonicates / T.M. Nicotera and G. Spehar -- Autofluorescent ceroid/lipofuscin / D. Yin and U. Brunk -- In vivo technique for autofluorescent lipopigments / F.C. Delori and C.K. Dorey -- An ex vivo erythrocyte model for investigating oxidative metabolism / L. Rossi and M. Magnani -- Purification of vesicular carriers from rat hepatocytes by magnetic immunoadsorption / L. Saucan -- Simultaneous determination of serum retinol, tocopherols, and carotenoids by HPLC / R.W. Browne and D. Armstrong -- EPR measurements of nitric oxide-induced chromanoxyl radicals of vitamin e. Interactions with vitamin c / V.E. Kagan and N.V. Gorbunov -- Nonvitamin plasma antioxidants / N.J. Miller -- Regulatory antioxidant enzymes / C.E. Pippenger, R.W. Browne and D. Armstrong -- In vitro screening for antioxidant activity / D. Armstrong, T. Hiramitsu and T. Ueda -- Antioxidant activity of low-density lipoprotein / N.J. Miller and G. Paganga -- Lipoic acid as an antioxidant. The role of dihydrolipoamide dehydrogenase / M.S. Patel and Y.S. Hong -- Reduced glutathione and glutathione disulfide / R.W. Browne and D. Armstrong -- Analysis of coenzyme Q10 content in human plasma and other biological samples / S. Graves [and others] -- A simple luminescence method for detecting lipid peroxidation and antioxidant activity in vitro / M. Nakano, T. Ito and T. Hiramitsu -- Analysis of oxidation and antioxidants using microtiter plates / G. Camejo, B. Wallin and M. Enojarvi -- Trace element analysis of biological samples by analytical atomic spectroscopy / H. Haraguchi, E. Fujimori and K. Inagaki -- Energy dispersive X-ray microanalysis / D.A. Samuelson -- Pro-oxidant and antioxidant effects of estrogens / J.G. Liehr and D. Roy -- Artificial radical generating and scavenging systems. Photo-fenton reagent and caged compounds / S. Matsugo and K. Fujimori -- Free radical and antioxidant protocols.

0

Text of Note

In Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. Free Radical and Antioxidant Protocols provides state-of-the-art methodology and biotechnology in a convenient format for both academic and corporate biomedical scientists. The detailed, laboratory-tested free-radical assays, many of them presented here for the first time, will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.

Text of Note

Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

Title

Free radical and antioxidant protocols.

International Standard Book Number

0896034720

Antioxidants-- Physiological effect, Laboratory manuals.

Free radicals (Chemistry)-- Pathophysiology, Laboratory manuals.

Oxidation, Physiological, Laboratory manuals.

Stress (Physiology)-- Research, Laboratory manuals.

Antioxidants-- metabolism.

Free Radicals-- metabolism.

Oxidative Stress-- physiology.

Antioxydants-- Effets physiologiques-- Manuels de laboratoire.

Oxydation biologique-- Manuels de laboratoire.

Radicaux libres (Chimie)-- Physiopathologie-- Manuels de laboratoire.

Stress-- Recherche-- Manuels de laboratoire.

Antioxidanten.

Antioxidants-- Physiological effect.

Free radicals (Chemistry)-- Pathophysiology.

MEDICAL-- Pathology.

MEDICAL-- Pathophysiology.

Oxidation, Physiological.

Radicalen (chemie)

Stress (Physiology)-- Research.

Wetenschappelijke technieken.

L800

MED-- 067000

MED-- 068000

PSB.

Number

Edition

Class number

Book number

Class number

Class number

Class number

Book Number

Book Number

System Code

Armstrong, Donald,1931-

Date of Transaction

20201208032642.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y