عنوان

MAP kinase signaling protocols /

(Number (ISBN

0896039986

(Number (ISBN

128035836X

(Number (ISBN

1592596711

(Number (ISBN

6610358362

(Number (ISBN

9780896039988

(Number (ISBN

9781280358364

(Number (ISBN

9781592596713

(Number (ISBN

9786610358366

Number

b708310

Title Proper

MAP kinase signaling protocols /

General Material Designation

[Book]

First Statement of Responsibility

edited by Rony Seger.

Place of Publication, Distribution, etc.

Totowa, N.J. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©2004.

Specific Material Designation and Extent of Item

1 online resource (xi, 332 pages) :

Other Physical Details

illustrations

Series Title

Methods in molecular biology ;

Volume Designation

v. 250

Text of Note

Includes index.

Text of Note

Includes bibliographical references and index.

Text of Note

The erk cascade as a prototype of mapk signaling pathways / H. Rubinfeld and R. Seger -- Determination of erk activity: Antiphospho-erk antibodies, in vitro phosphorylation, and in-gel kinase assay / S. Kraus and R. Seger -- Detection of erk1/2 activities using affinity reagents / R. Pulido, A. Zuniga and A. Ullrich -- Activation of sapks/jnks and p38s in vitro / J.M. Kyriakis, H. Liu and D.N. Chadee -- Investigating the cellular bmk1/erk5 signaling pathway / R.I. Tapping [and others] -- Pull-down assays for guanoside 5'-triphosphate-bound ras-like guanosine 5'-triphosphatases / M. van Triest and J.L. Bos -- Regulation of mapk cascades by protein tyrosine phosphatases / J. Torres, C. Blanco-Aparicio and R. Pulido -- Use of inhibitors in the study of mapk signaling / Y.D. Shaul and R. Seger -- Structure of mapks / E.J. Goldsmith, M.H. Cobb and C.I. Chang -- Subcellular localization of mapks / M. Adachi and E. Nishida -- Study of mapk signaling using knockout mice / G. Pages and J. Pouyssegur -- Computer simulation of mapk signal transduction / B.D. Aguda and H.M. Sauro -- Signaling by growth factor receptors / B.S. Kochupurakkal and Y. Yarden -- Activation of mapks by g protein-coupled receptors / P. Crespo and J.S. Gutkind -- Identification of mapk substrates by expression screening with solid-phase phosphorylation / R. Fukunaga and T. Hunter -- Study of substrate specificity of mapks using oriented peptide libraries / M.B. Yaffe -- Ras signaling pathway for analysis of protein-protein interactions in yeast and mammalian cells / A. Aronheim -- Methods in functional proteomics: Two-dimensional polyacrylamide gel electrophoresis with immobilized ph gradients, in-gel digestion and identification of proteins by mass spectrometry / K.R. Bernard [and others] -- Practical methods for deuterium exchange/mass spectrometry / A.N. Hoofnagle, K.A. Resing and N.G. Ahn -- Use of xenopus oocytes and early embryos to study mapk signaling / E. Perdiguero and A.R. Nebreda -- Mapk cascades in the brain: Lessons from learning / D.E. Berman and Y. Dudai.

0

Text of Note

Mitogen-activated protein kinase (MAPK) signaling cascades initiate highly significant cellular processes and are involved in a large number of diseases, including cancer and diabetes. In MAP Kinase Signaling Protocols, leading researchers with extensive hands-on experience describe in detail both classic and cutting-edge techniques for the detection and measurement of the MAPKs and other components in these cascades. The protocols include methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the downregulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and versatile, MAP Kinase Signaling Protocols offers biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.

Stock Number

springerp

Title

MAP kinase signaling protocols.

International Standard Book Number

0896039986

Cellular signal transduction-- Research-- Methodology.

Mitogen-activated protein kinases-- Research-- Methodology.

MAP Kinase Signaling System

Signal Transduction-- physiology

Cellular signal transduction-- Research-- Methodology.

SCIENCE-- Life Sciences-- Biochemistry.

PSB.

SCI-- 007000

Number

Edition

Class number

Book number

Class number

Class number

Book Number

Book Number

Seger, Rony.

Date of Transaction

20201207231700.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y