Functionalization -- Non-covalent attachment of proteins to single-walled carbon nanotubes / Luís F.F. Neves [and others] -- Covalent conjugation of multi-walled carbon nanotubes with proteins / Changqing Yi [and others] -- Covalently linked deoxyribonucleic acid with multi-walled carbon nanotubes: synthesis and characterization / Weiwei Chen [and others] -- Temperature and pH-responsive "smart" carbon nanotube dispersions / Dan Wang and Liwei Chen -- Toxicity -- Effects of carbon nanotubes on the proliferation and differentiation -primary osteoblasts / Dawei Zhang [and others] -- Carbon nanotube uptake and toxicity in the brain / Leying Zhang, Darya Alizadeh, and Behnam Badie -- In Vitro and in vivo biocompatibility testing of functionalized carbon nanotubes / Gianni Ciofani [and others] -- Real-time monitoring of cellular responses to carbon nanotubes / Qingxin Mu, Shumei Zhai, and Bing Yan -- Reducing nanotube cytotoxicity using a nano-combinatorial library approach / Qiu Zhang, Hongyu Zhou, and Bing Yan -- DNA damage by carbon nanotubes using the single cell gel electrophoresis technique / Olga Zeni and Maria Rosaria Scarfì -- Trafficking -- Assessment of cellular uptake and cytotoxicity of carbon nanotubes using flow cytometry / Khuloud T. Al-Jamal and Kostas Kostarelos -- Cell trafficking of carbon nanotubes based on fluorescence detection / Monica H. Lamm and Pu Chun Ke -- Carbon nanotubes as intracellular carriers for multidrug resistant cells studied by capillary electrophoresis-laser-induced fluorescence / Ruibin Li [and others] -- Scaffolds -- Carbon nanotube-based neurochips / Moshe David-Pur, Mark Shein, and Yael Hanein -- Effect of carbon nanotubes on HepG2 adhesion and spreading / Suijian Qi [and others] -- Biosensors -- Enzymatic detection based on carbon nanotubes / Martin Pumera -- Carbon nanotube biosensors based on electrochemical detection / Martin Pumera -- Biosensors based on carbon nanotube-network field-effect transistors / Cristina C. Cid [and others] -- Detection of biomarkers with carbon nanotube-based immunosensors / Samuel Sánchez, Esteve Fàbregas, and Martin Pumera -- Carbon nanotube biosensors with aptamers as molecular recognition elements / Hye-Mi So [and others].
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SUMMARY OR ABSTRACT
Text of Note
Due to their rare combination of high chemical stability, exceptional optical and electrical properties, high surface-to-volume ratio, and high aspect ratio, carbon nanotubes (CNTs) have made an enormous impact on materials science, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: Methods and Protocols provides reliable, consistent protocols on the application of CNTs in molecular biology-related fields. These are of vital importance, as the commercially available CNTs differ in purity, agglomeration state, as well as length and diameter distribution, all of which have a profound influence on the dispersability and surface properties of the tubes. The volume contains detailed sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, provided by expert researchers from various fields. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Carbon Nanotubes: Methods and Protocols serves to contribute to the achievement of common standards and helps researchers to avoid discrepancies in future biology-related CNT studies.