Preliminary Evaluation of the Single-Tree, Huanglongbing Find in California
General Material Designation
[Article]
First Statement of Responsibility
Wang, J.; Roose, M.; Ramadugu, C.; Lee, R.; Manjunath, K.; Lin, H.; Chen, J.; Shatters, R.; Polek, M.; LeVesque, C.; Vidalakis, G.
SUMMARY OR ABSTRACT
Text of Note
Huanglongbing (HLB, citrus greening) associated with 'Candidatus Liberibacter' species is a widespread devastating citrus disease not previously reported in California (CA). In March 2012, 'C. Liberibacter asiaticus' (CLas) was detected from an Asian citrus psyllid (ACP, Diaphorina citri) sample from Los Angeles, CA at the Jerry Dimitman Laboratory of the Citrus Research Board. Subsequent citrus plant surveys within a 400m area of the CLas-positive ACP sample performed by the California Department of Food and Agriculture identified an infected multi-grafted citrus tree at a residence in Los Angeles, CA. The CLas-positive tree was removed and nucleic acids from different plant tissues (i.e. roots, trunk, stems, and leaves) were extracted and distributed to several federal, state, and university laboratories nationwide for preliminary evaluation. Labs attempted to identify the species and graft types of the infected citrus, study the genetic characteristics and genome diversity of the detected bacterium, as well as test for other graft-transmissible citrus pathogens (GTCP). Preliminary data suggested that one type of lemon was the original rootstock that received over 20 citrus grafts. CLas DNA population analysis suggested a possible single Asian origin. Preliminary tests indicated the possible presence of other GTCP. Further evaluations on the CA CLas-positive find are ongoing.
SET
Date of Publication
2014
Title
Journal of Citrus Pathology
Volume Number
1
PERSONAL NAME - PRIMARY RESPONSIBILITY
Entry Element
Wang, J.; Roose, M.; Ramadugu, C.; Lee, R.; Manjunath, K.; Lin, H.; Chen, J.; Shatters, R.; Polek, M.; LeVesque, C.; Vidalakis, G.