عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
NATIONAL BIBLIOGRAPHY NUMBER
Number
LA74z341mr
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
A minimalist biosensor:
General Material Designation
[Article]
First Statement of Responsibility
Kellenberger, Colleen A; Sales-Lee, Jade; Pan, Yuchen; Gassaway, Madalee M; Herr, Amy E; Hammond, Ming C
Title Proper by Another Author
Quantitation of cyclic di-GMP using the conformational change of a riboswitch aptamer.
SUMMARY OR ABSTRACT
Text of Note
Cyclic di-GMP (c-di-GMP) is a second messenger that is important in regulating bacterial physiology and behavior, including motility and virulence. Many questions remain about the role and regulation of this signaling molecule, but current methods of detection are limited by either modest sensitivity or requirements for extensive sample purification. We have taken advantage of a natural, high affinity receptor of c-di-GMP, the Vc2 riboswitch aptamer, to develop a sensitive and rapid electrophoretic mobility shift assay (EMSA) for c-di-GMP quantitation that required minimal engineering of the RNA.
SET
Date of Publication
2015
Title
UC Berkeley
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب [Article]
275578
a
Y
