عنوان

Engineering of a light-activated Caspase-3 in vitro and in Drosophila neurons

Number

TL4zg5b6mg

.Language of Text, Soundtrack etc

انگلیسی

Title Proper

Engineering of a light-activated Caspase-3 in vitro and in Drosophila neurons

General Material Designation

[Thesis]

First Statement of Responsibility

Smart, Ashley Danielle

Subsequent Statement of Responsibility

Davis, Graeme W; Wells, James A

Name of Publisher, Distributor, etc.

UCSF

Date of Publication, Distribution, etc.

2017

Body granting the degree

UCSF

Text preceding or following the note

2017

Text of Note

A cell is a complex structure filled with proteins that are undertaking hundreds of tasks. Some proteins have multiple different roles within a cell. These proteins must be differentially controlled temporally and/or spatially so the cell can use them to achieve different functional outcomes. Many mechanisms could be used to control these proteins, but we currently do not know how different cell regulation mechanisms lead to different protein roles. To this end, we have engineered one protein with temporal and spatial control to have a tool to begin to understand how different protein regulations lead to different outcomes. The protein we engineered is Caspase-3 which has a variety of roles from apoptosis to synaptic remodeling. This dissertation provides the strategy used to create a light-activated caspase and the testing and optimization of it in vitro and in vivo. Chapter 1 discusses the background of caspases as well as their different roles in cells. Chapter 2 provides the design of the light-activated caspase including in silico modelling and testing in vitro. Chapter 3 discusses the testing of the light-activated caspase in vivo and our findings that different neuronal cell types respond differently to caspase activation. Chapter 4 discusses potential modifications and improvements to the light-activated caspase and how a light-activated caspase can be used to further understand what type of regulation of proteins can lead to different cellular outcomes.

Smart, Ashley Danielle

Davis, Graeme W; Wells, James A

UCSF

Electronic name

p

[Thesis]

276903

a

Y