عنوان

3D surface topology guides stem cell adhesion and differentiation.

Number

LA9b74g3vx

Title Proper

3D surface topology guides stem cell adhesion and differentiation.

General Material Designation

[Article]

First Statement of Responsibility

Viswanathan, PriyalakshmiOndeck, Matthew GChirasatitsin, SomyotNgamkham, KamolchanokReilly, Gwendolen CEngler, Adam JBattaglia, Giuseppe

Text of Note

Polymerized high internal phase emulsion (polyHIPE) foams are extremely versatile materials for investigating cell-substrate interactions in vitro. Foam morphologies can be controlled by polymerization conditions to result in either open or closed pore structures with different levels of connectivity, consequently enabling the comparison between 2D and 3D matrices using the same substrate with identical surface chemistry conditions. Additionally, here we achieve the control of pore surface topology (i.e. how different ligands are clustered together) using amphiphilic block copolymers as emulsion stabilizers. We demonstrate that adhesion of human mesenchymal progenitor (hES-MP) cells cultured on polyHIPE foams is dependent on foam surface topology and chemistry but is independent of porosity and interconnectivity. We also demonstrate that the interconnectivity, architecture and surface topology of the foams has an effect on the osteogenic differentiation potential of hES-MP cells. Together these data demonstrate that the adhesive heterogeneity of a 3D scaffold could regulate not only mesenchymal stem cell attachment but also cell behavior in the absence of soluble growth factors.

Date of Publication

2015

Title

UC San Diego

Electronic name

[Article]

277679

a

Y