Study of bacteriorhodopsin in a controlled lipid environment /
General Material Designation
[Book]
First Statement of Responsibility
Vivien Yeh.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
Singapore :
Name of Publisher, Distributor, etc.
Springer,
Date of Publication, Distribution, etc.
[2019]
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
1 online resource
SERIES
Series Title
Springer theses
GENERAL NOTES
Text of Note
"Doctoral thesis accepted by National Taiwan University, Taipei, Taiwan."
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references.
CONTENTS NOTE
Text of Note
Introduction -- Experimental Background -- Effect of Lipid Composition of Nanodisc -- Effect of Size of Nanodisc -- Native Membrane Nanodisc -- Conclusions and Outlook -- References -- Appendix.
0
SUMMARY OR ABSTRACT
Text of Note
This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.
ACQUISITION INFORMATION NOTE
Source for Acquisition/Subscription Address
Springer Nature
Stock Number
com.springer.onix.9789811312380
OTHER EDITION IN ANOTHER MEDIUM
Title
Study of bacteriorhodopsin in a controlled lipid environment.