4 NotesReferences; Chapter 5: Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain Using Preformed Fibrils; 1 Introduction; 2 Materials; 2.1 Sonication of aSyn PFFs; 2.2 Stereotaxic Injection into Mouse Brain; 2.3 Mouse Perfusion and Confirmation of Pathology by Immunohistochemistry; 3 Methods; 3.1 Sonication of aSyn PFFs; 3.2 Stereotaxic Injection into Mouse Brain; 3.3 Coordinates for Different CNS Sites; 3.4 Mouse Perfusion and Confirmation of Pathology by Immunohistochemistry; 3.5 Decontamination of Instruments and Work Surfaces; 4 Notes; References
Text of Note
Chapter 6: Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection1 Introduction; 2 Materials; 3 Methods; 3.1 Preparation of Biospecimen; 3.2 SULFO-TAG Labeling of Detection Antibodies; 3.3 Coating of Assay Plates; 3.4 Washing; 3.5 Blocking; 3.6 Preparation of Standard Curves; 3.7 Sample Dilution; 3.8 Application of Standards and Samples to the Assay Plate; 3.9 Application of Detection Antibody; 3.10 Measurement; 3.11 Data Analysis; 4 Notes; References