عنوان

Mouse oocyte development :

(Number (ISBN

1493986031

(Number (ISBN

9781493986033

Erroneous ISBN

1493986023

Erroneous ISBN

9781493986026

Title Proper

Mouse oocyte development :

General Material Designation

[Book]

Other Title Information

methods and protocols /

First Statement of Responsibility

edited by Marie-Hélène Verlhac, Marie-Emilie Terret.

Place of Publication, Distribution, etc.

New York, NY :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

[2018]

Date of Publication, Distribution, etc.

©2018

Specific Material Designation and Extent of Item

1 online resource (xi, 174 pages) :

Other Physical Details

illustrations (some color)

Series Title

Methods in molecular biology,

Volume Designation

volume 1818

ISSN of Series

1940-6029 ;

Text of Note

Includes bibliographical references and index.

Text of Note

Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse -- In vitro Culture of Mouse Oocytes for Meiotic Maturation -- In vitro Maturation and Culture of Human Oocytes -- Pronuclear Transfer in Human Oocytes -- In vitro Fertilization of Mouse Oocyte -- Profiling Maternal mRNA Translation during Oocyte Development -- Transcriptome Profiling of Single Mouse Oocytes -- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation -- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation -- Triple-colour Live Imaging of Mouse Oocytes -- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes -- Manipulating Cohesin Levels in Live Mouse Oocytes -- Optogenetic Manipulation of Mouse Oocytes -- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin -- Photoactivation of Actin in Mouse Oocyte -- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes -- Micropipette Aspiration of Oocytes to Assess Cortical Tension.

0

Text of Note

This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.

Source for Acquisition/Subscription Address

Springer Nature

Stock Number

com.springer.onix.9781493986033

Title

Mouse oocyte development.

International Standard Book Number

9781493986026

Mice-- Reproduction, Laboratory manuals.

Ovum, Laboratory manuals.

In Vitro Oocyte Maturation Techniques.

Mice-- embryology.

Oocytes-- growth & development.

Cellular biology (cytology)

Mice-- Reproduction.

Ovum.

Science-- Life Sciences-- Biology-- Molecular Biology.

Number

Edition

Class number

Class number

Class number

Book Number

Terret, Marie-Emilie

Verlhac, Marie-Hélène

Date of Transaction

20200823034545.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y