عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
NATIONAL BIBLIOGRAPHY NUMBER
Number
TLets794350
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
Fluorescence probes for the detection of biologically relevant species
General Material Designation
[Thesis]
First Statement of Responsibility
Gardiner, Jordan
Subsequent Statement of Responsibility
James, Tony ; Van Den Elsen, Johannes
.PUBLICATION, DISTRIBUTION, ETC
Name of Publisher, Distributor, etc.
University of Bath
Date of Publication, Distribution, etc.
2019
DISSERTATION (THESIS) NOTE
Dissertation or thesis details and type of degree
Thesis (Ph.D.)
Text preceding or following the note
2019
SUMMARY OR ABSTRACT
Text of Note
Detection of chemical species in physiological systems is of great importance in the research and investigation of human diseases. Fluorescence spectroscopy has proven itself as an essential tool for the study of targets in biological systems. It is a reliable method for the semi-quantitative analysis of target analytes and provides a near unprecedented level of spatial and temporal resolution. As such, there is a need for increased specificity and new modes of detection within the field of fluorescent probes which drives research and development in the area. An introduction to the background of fluorescence is given herein, with explanations of the common mechanisms of fluorescence. Then, analytes that are commonly targeted for detection and probes that have been designed to target them are exemplified. Three research chapters follow, describing the design, synthesis and analysis of new probes developed throughout this thesis. Coumarin, fluorescein and TCF fluorophores are used ascore units in each of the chapters formed. Probes that have been developed include MLGsfor dual analyte "AND" detection, that require the presence of two species for a signal tobe released. A coumarin-based triple analyte "AND" probe has also been developed thatrequired the presence of Glutamate, Zinc2+ and peroxynitrite (ONOO-), which has thepotential to detect diseased neurons in the brain. Single analyte TCF-based probes havealso been developed for the detection of ONOO and glutathione. A number of theseprobes have been studied in cellular systems and show great promise for use in the study ofhuman disease and revealing important information between the relationships of chemicals therein.
TOPICAL NAME USED AS SUBJECT
Fluorescent Probes ; Molecular Logic Gates
PERSONAL NAME - PRIMARY RESPONSIBILITY
Gardiner, Jordan
PERSONAL NAME - SECONDARY RESPONSIBILITY
James, Tony ; Van Den Elsen, Johannes
CORPORATE BODY NAME - SECONDARY RESPONSIBILITY
University of Bath
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب p
[Thesis]
276903
a
Y
