Application of Recombinant Baculoviruses in Biopharmaceutical Research --; Engineering Post-Translational Processing of Recombinant Proteins Produced in Insect Cell Culture --; A Guide to Successful Scale-up of the Baculovirus Expression System --; Transient Gene Expression In Mammalian Cells Based on the Calcium Phosphate Transfection Method --; Adenovirus Vectors in Functional Genomics --; Adeno-Associated Virus: A Promising Tool For Gene Delivery --; Alphavirus Vectors: Highly Efficient Systems for Transient Gene Expression --; Transient Expression in Mammalian Cells: Applications and Perspectives.
SUMMARY OR ABSTRACT
Text of Note
This volume focuses on the transient gene expression technologies for recombinant protein production and gene therapy. It provides coverage of an important issue in Cell Engineering by internationally authoritative experts. Topics covered range from the use of baculovirus, adenovirus, adeno-associated virus and alphavirus vectors in biopharmaceutical, genomics and gene delivery research, to large-scale transfection of plasmid DNA into cultured cells for bioreactor applications. This book illuminates all the scientific and technological issues involved in the research, development, production, and human use of viral and non-viral vectors for in vitro and in vivo expression of recombinant proteins, thus making it an essential reference for cell culture technologists, virologists, and scientists interested in gene therapy.