edited by Stanton B. Gelvin, Robbert A. Schilperoort.
EDITION STATEMENT
Edition Statement
Second edition
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
Dordrecht
Name of Publisher, Distributor, etc.
Springer Netherlands
Date of Publication, Distribution, etc.
2000
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
(xxi, 161 pages)
CONTENTS NOTE
Text of Note
E3: Yeast as versatile tool in transcription factor research --; H5: The construction of bacterial artificial chromosome (BAC) libraries --; N1: Gene transfer mediated by site-specific recombination systems --; N2: Use of the R-RS site-specific recombination system in plants --; N3: Cre-lox directed integration of transgenes into the tobacco genome --; 01: Plant actin isolation and characterization.
SUMMARY OR ABSTRACT
Text of Note
Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.
TOPICAL NAME USED AS SUBJECT
Botany.
Life sciences.
Plant physiology.
LIBRARY OF CONGRESS CLASSIFICATION
Class number
QK711
.
2
Book number
E358
2000
PERSONAL NAME - PRIMARY RESPONSIBILITY
edited by Stanton B. Gelvin, Robbert A. Schilperoort.