Machine generated contents note: Preface vii --;Contributors ix --;1 Protein Engineering in Basic and Applied Biotechnology: A Review 1 --;Lilia Alberghina and Marina Lotti --;2 Enhanced Recovery and Folding of Recombinant Proteins Using --;Fusion Protein Strategies 23 --;Sophia Hober and Matlias Uhlen --;3 Protein Engineerinng for Afftriity Pf-ifcation: Thl Strep-tag 41 --;Thomas G.M. Schmia. and Arne Skerra, --;4 Candida rugosa Lipases: From Molecular'Evolution Analysis to the --;Design of a Synthetic Gene 63 --;Marina Lotti and Lilia Alberghina --;5 Structure-Function Studies on Cutinase, a Small Lipolytic Enzyme --;with a Water Accessible Active Site 75 --;Sonia Longhi, Anne Nicolas, Christian Jelsch and Christian Cambillau --;6 Lipases from Rhizopus Species: Genetics, Structure and Applications 115 --;Uwe T Bornscheuer, Jiirgen Pleiss, Claudia Schmidt-Dannert and --;RolfD. Schmid --;7 Solvent Engineering Modulates Stereoselectivity of Microbial Lipases 135 --;Enrico Cernia, Cleofe Palocci and Simonetta Soro --;8 Stabilization of Industrial Enzymes by Protein Engineering 147 --;Guido Grandi, Imma Margarit, Francesco Frigerio, Renzo Nogarotto, --;Giovanna Carpani, Renata Grifantini and Giuliano Galli --;9 Design of Biotechnologically Applicable Yeast Carboxypeptidase --;Y Mutants with Increased Preference for Charged P1 Residues 159 --;Kjeld Olesen and Klaus Breddam --;10 Engineering 3-Glycoside Hydrolases 169 --;RA. J. Warren --;11 Engineering Specificity and Stability in Glucoamylase from --;Aspergillus niger 189 --;Torben P. Frandsen, Henri-Pierre Fierobe and Birte Svensson --;12 Pectin Modifying Enzymes and Designer Pectins 207 --;Olga Mayans, John Jenkins, Drummond Smith, Kathryn Worboys and --;Richard Pickersgill.