عنوان

Cornea in Normal Condition and in Groenouw's Macular Dystrophy.

(Number (ISBN

9400991770

(Number (ISBN

9789400991774

Number

b542907

Title Proper

Cornea in Normal Condition and in Groenouw's Macular Dystrophy.

General Material Designation

[Book]

First Statement of Responsibility

Jules Franáois

Place of Publication, Distribution, etc.

Dordrecht

Name of Publisher, Distributor, etc.

Springer Netherlands

Date of Publication, Distribution, etc.

1980

Text of Note

one: Normal keratocytes.- I: Material and methods.- I. Material.- II. Methods.- A. Histological and histochemical techniques.- B. Culture techniques for normal keratocytes.- C. Technique for the study of the corneal architectonic.- D. Histochemical techniques.- III. Discussion.- A. Mucopolysaccharides.- B. Collagen and reticulin.- C. Lysosomes.- D. Mitochondria.- IV. Microscopy.- V. Photography.- II: Normal keratocytes in situ.- I. Introduction.- II. Personal results.- Discussion and conclusions.- III: Architectonic of the cornea.- I. Introduction.- II. Personal results.- A. Flat sections.- B. Transversal sections.- C. Study of flat sections at the polarisation microscope.- D. Study of the transversal sections at the polarisation microscope.- E. Study of the mucopolysaccharides.- F. Topo-optical study.- G. Diffraction by X-rays.- H. Examination of collagen-mucopolysaccharide complexes at the high-resolution electron micoscope.- I. Macromolecular model of the corneal stroma.- Discussion.- IV: The keratocytes in the process of cicatrisation of the corneal stroma.- I. Personal observations.- A. Transformation of the keratocytes in a wound.- B. Histochemical evolution of the keratocytes in the course of cicatrisation.- Discussion and conclusions.- V: Keratocytes in tissue culture.- I. Introduction.- II. Personal observations.- A. Examination of the primary cultures in vivo during growth.- B. Examination of the sub-cultures in vivo during growth.- C. Examination at the phase-contrast microscope of living monolayers, mounted in Ringer's solution.- III. Histochemical study.- IV. Electron microscopy.- V. Scanning microscopy.- Discussion and conclusions.- two: Pathological keratocytes (Macular dystrophy of the cornea).- VI.- I. Definition of macular dystrophy of the cornea.- II. Clinical characteristics.- III. Heredity.- Conclusions.- VII: Microscopical and histochemical study of macular dystrophy.- Personal observations.- A. Phase-contrast microscopy of fresh sections and of sections fixed before and after dehydration.- B. Optical microscopy of histochemically stained sections.- C. Polarisation microscopy and topo-optical staining.- D. Dark-field microscopy.- Discussions and conclusions.- VIII: Transmission electron microscopy of the corneal stroma in macular dystrophy of the cornea.- Personal observations.- A. Superficial layers of the corneal stroma.- B. Keratocytes.- I. First stage of the keratocyte degeneration.- II. Second stage of the keratocyte degeneration.- III. Third stage of the keratocyte degeneration.- IV. Fourth and final stage of the keratocyte degeneration.- C. Intercellular corneal stroma.- Discussions and conclusions.- IX: Scanning electron microscopy of the corneal stroma in macular dystrophy.- Personal observations.- Conclusion.- X: Changes of the epithelium, the base membrane, the endothelium and the Descemet's membrane in macular dystrophy of the cornea.- Personal observations.- I. Deterioration of the epithelium and the base membrane.- A. Optical microscopy and histochemistry.- a. Examination at the clear-field microscope of stained sections.- b. Phase-contrast microscopy of fresh or histochemically stained sections.- c. Polarisation microscopy of histochemically stained sections.- d. Dark-field microscopy of stained sections.- B. Electron microscopy.- C. Scanning electron microscopy.- II. Changes of the endothelium and the Descemet's membrane.- Discussion.- Conclusion.- XI: Histochemical and microscopical study of corneal cultures from macular dystrophy.- Personal observations.- I. Development of the cultures.- II. Examination of the implantation specimen.- III. Microscopy.- A. Implanted fragment.- B. Keratocytes in monolayer.- Discussion.- Conclusions.- XII: Transmission electron microscopy of cultures of the corneal stroma in macular dystrophy.- Personal observations.- Discussion.- Conclusions.- XIII: Scanning electron microscopy of cultures of macular dystrophy of the cornea.- Personal observations.- Comparative study of normal keratocytes and keratocytes affected by macular dystrophy.- Conclusions.- XIV: Vital staining of the lysosomes in normal keratocytes and in keratocytes from macular dystrophy.- Personal observations.- I. Normal keratocytes.- Cell division.- Dynamics of the vital staining.- II. Keratocytes in macular dystrophy.- Dynamics of the vital staining in keratocytes from macular dystrophy.- Conclusions.- XV: Active biological substance present in normal keratocytes and capable of acting on the stored mucopolysaccharides in macular dystrophy of the cornea.- Personal observations.- I. Experiment.- II. Results.- Discussion and conclusions.- XVI: Pathogenesis of macular dystrophy of the cornea.- XVII: Treatment of macular dystrophy of the cornea.- Summary.- Summary.

Class number

Book number

Jules Franáois

Jules Franáois

Virgilio Victoria-Troncoso

Electronic name

[Book]

Y