عنوان

Immunocytochemical methods and protocols

(Number (ISBN

1588294633 (alk. paper)

(Number (ISBN

9781588294630 (alk. paper)

Number

b517381

Title Proper

Immunocytochemical methods and protocols

General Material Designation

[Book]

Edition Statement

3rd ed. /

Statement of Responsibility Relating to Edition

edited by Constance Oliver and Maria Célia Jamur

Place of Publication, Distribution, etc.

New York, NY :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

c2010

Specific Material Designation and Extent of Item

xii, 416 p. :

Other Physical Details

ill. ;

Dimensions

27 cm

Series Title

Methods in molecular biology,

Series Title

Springer protocols

Volume Designation

v. 588

ISSN of Series

1064-3745 ;

Text of Note

Previous ed.: 1999

Text of Note

Includes bibliographical references and index

Text of Note

Overview of antibodies for immunochemistry / Constance Oliver and Maria Célia Jamur -- Introduction to the purification of antibodies / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : ammonium sulfate fractionation or gel filtration / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : ion-exchange chromatography / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : affinity chromatography--Protein A and Protein G Sepharose / Ana Cristina Grodzki and Elsa Berenstein -- Conjugation of fluorochromes to antibodies / Su-Yau Mao and J. Michael Mullins -- Biotinylation of antibodies / Su-Yau Mao -- Cell fixatives for immunostaining / Maria Célia Jamur and Constance Oliver -- Permeabilization of cell membranes / Maria Célia Jamur and Constance Oliver -- Preparation of frozen sections for analysis / Gary L. Bratthauer -- Processing of cytological specimens / Gary L. Bratthauer -- Processing of tissue culture cells / Gary L. Bratthauer -- Processing of tissue specimens / Gary L. Bratthauer -- Heat induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections / Laszlo Krenacs, Tibor Krenacs, Eva Stelkovics, and Mark Raffeld -- Fluorochromes : properties and characteristics / J. Michael Mullins -- Direct immunofluorescent labeling of cells / Maria Veronica Dávila Pástor -- Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells / Mark C. Willingham -- Fluorescence labeling of intracellular antigens of attached or suspended tissue-culture cells / Mark C. Willingham -- Fluorescent visualization of macromolecules in Drosophila whole mounts / Ricardo Guelerman Pinheiro Ramos, Luciana Claudia Herculano Machado, and Livia Maria Rosatto Moda -- Overview of conventional fluorescence photomicrography / J. Michael Mullins -- Overview of confocal microscopy / William D. Swaim -- Overview of laser microbeam applications as related to antibody targeting / P. Scott Pine -- Immuno-laser capture microdissection of rat brain neurons for real time quantitative PCR / Denis G. Baskin and L. Scot Bastian -- Overview of antigen detection through enzymatic activity / Gary L. Bratthauer -- The peroxidase-antiperoxidase (PAP) method and other all-immunologic detection methods / Gary L. Bratthauer -- The avidin-biotin complex (ABC) method and other avidin-biotin binding methods / Gary L. Bratthauer -- Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue / Mark Raffeld and Elaine S. Jaffe -- Multiple antigen immunostaining procedures / Tibor Krenacs, Laszlo Krenacs, and Mark Raffeld -- Immunoenzymatic quantitative analysis of antigens expressed on the cell surface (cell-ELISA) / Elaine Vicente Lourenço, Maria-Cristina Roque-Barreira -- Use of immunogold with silver enhancement / Constance Oliver -- Overview of flow cytometry and fluorescent probes for flow cytometry / Robert E. Cunningham -- Tissue disaggregation / Robert E. Cunningham -- Indirect immunofluorescent labeling of viable cells / Robert E. Cunningham -- Indirect immunofluorescent labeling of fixed cells / Robert E. Cunningham -- Fluorescent labeling of DNA / Robert E. Cunningham -- Deparaffinization and processing of pathologic material / Robert E. Cunningham -- Fixation and embedding / Constance Oliver and Maria Célia Jamur -- Preparation of colloidal gold / Constance Oliver -- Conjugation of colloidal gold to proteins / Constance Oliver -- Colloidal gold/streptavidin methods / Constance Oliver -- Pre-embedding labeling methods / Constance Oliver -- Postembedding labeling methods / Constance Oliver -- The Clinical immunohistochemistry laboratory : regulations and troubleshooting guidelines / Patricia A. Fetsch and Andrea Abati

0

Immunocytochemistry, Laboratory manuals

In situ hybridization, Laboratory manuals

Immunohistochemistry-- methods

Immunohistochemistry-- standards

Number

Class number

Jamur, Maria Célia

Oliver, Constance

Date of Transaction

20100318011131.0

Electronic name

[Book]

Y