عنوان

Protein chromatography :

(Number (ISBN

1607619121

(Number (ISBN

9781607619123

Number

b507456

Title Proper

Protein chromatography :

General Material Designation

[Book]

Other Title Information

methods and protocols /

First Statement of Responsibility

edited by Dermot Walls and Sinéad T. Loughran.

Place of Publication, Distribution, etc.

New York, N.Y. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

c2011.

Specific Material Designation and Extent of Item

x, 527 p. :

Other Physical Details

ill. (some col.) ;

Dimensions

27 cm.

Series Title

Methods in molecular biology,

Series Title

Springer protocols

Volume Designation

v. 681

ISSN of Series

1940-6029 ;

Text of Note

Includes bibliographical references and index.

Text of Note

A digest of protein purification -- Gel-filtration chromatography -- Immunoaffinity chromatography -- Avoiding proteolysis during protein chromatography -- Scale-up of protein purification : Downstream processing issues -- Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources -- Engineering protein stability -- Microfluidics in protein chromatography -- Tagging recombinant proteins to enhance solubility and aid purification -- Storage and lyophilisation of pure proteins -- Differential precipitation and solubilization of proteins -- Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase -- Protein quantitation and analysis of purity -- Purification of proteins fused to glutathione S-transferase -- Purification of proteins fused to maltose-binding protein -- Purification of proteins from baculovirus-infected insect cells -- Purification of poly-histidine-tagged proteins -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry -- Tag removal by site-specific cleavage of recombinant fusion proteins -- Purification of antibodies using affinity chromatography -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens -- Measuring protein-protein interactions using biacore -- Ultra-performance liquid chromatography-mass spectrometry of proteins -- Hydrophobic interaction chromatography -- Fast protein liquid chromatography -- Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS -- Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS -- Clinical proteomics : Liquid chromatography-mass spectrometry purification systems -- Strategies for the purification of membrane proteins -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases.

0

Title

Protein chromatography.

International Standard Book Number

9781607619130

Chromatographic analysis.

Proteins-- Analysis.

Proteomics.

Chromatography-- methods.

Proteins-- analysis.

Loughran, Sinéad T.

Walls, Dermot.

Date of Transaction

20101216081829.0

Electronic name

[Book]

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