Part I Detection, Isolation, and Characterization of Quorum Sensing Compounds -- Bioassays of Quorum Sensing Compounds Using Agrobacterium tumefaciens and Chromobacterium violaceum / Weihua Chu, Dhiraj A. Vattem, Vatsala Maitin, Mary B. Barnes, and Robert J.C. McLean -- Detection of 2-Alkyl-4-Quinolones Using Biosensors / Stephen P. Diggle, Matthew P. Fletcher, Miguel Cámara, and Paul Williams -- FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds / Sathish Rajamani and Richard Sayre -- Isolation of agr Quorum Sensing Autoinducers / Naomi Balaban -- Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of N-Acyl-l-Homoserine Lactones and 4-Hydroxy-2-Alkylquinolines / François Lépine and Eric Déziel -- Detection of Autoinducer (AI-2)-Like Activity in Food Samples / Kirthiram K. Sivakumar, Palmy R. Jesudhasan, and Suresh D. Pillai -- Detection of Bacterial Signaling Molecules in Liquid or Gaseous Environments / Peter Edmonson, Desmond Stubbs, and William Hunt -- Determination of Acyl Homoserine Lactone and Tetramic / Acid Concentrations in Biological Samples / Colin A. Lowery, Gunnar F. Kaufmann, and Kim D. Janda -- Luminescent Reporters and Their Applications for the Characterization of Signals and Signal-Mimics that Alter LasR-Mediated Quorum Sensing / Ali Alagely, Sathish Rajamani, and Max Teplitski -- Part II Determining the Function of Autoinducers In vivo -- Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules / Vladimir V. Kravchenko, Richard J. Ulevitch, and Gunnar F. Kaufmann -- Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo / Louise Dahl Christensen, Maria van Gennip, Tim Holm Jakobsen, Michael Givskov, and Thomas Bjarnsholt -- Defining the Structure and Function of Acyl-Homoserine Lactone Autoinducers / Mair E.A. Churchill, Hiruy M. Sibhatu, and Charis L. Uhlson -- Global Expression Analysis of Quorum-Sensing Controlled Genes / Martin Schuster -- Small RNA Target Genes and Regulatory Connections in the Vibrio cholerae Quorum Sensing System / Brian K. Hammer and Sine Lo Svenningsen -- Quantifying Pseudomonas aeruginosa Quinolones and Examining Their Interactions with Lipids / Gregory C. Palmer, Jeffrey W. Schertzer, Lauren Mashburn-Warren, and Marvin Whiteley -- Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans / Aurélie Deveau and Deborah A. Hogan -- Design of Synthetic Mammalian Quorum-Sensing Systems / Wilfried Weber and Martin Fussenegger -- Part III Quorum Sensing Disruption Strategies -- Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro / Tim Holm Jakobsen, Maria van Gennip, Louise Dahl Christensen, Thomas Bjarnsholt, and Michael Givskov -- Custom Synthesis of Autoinducers and Their Analogues / Jun Igarashi and Hiroaki Suga -- Heterologous Overexpression, Purification, and In Vitro Characterization of AHL Lactonases / Pei W. Thomas and Walter Fast -- High-Performance Liquid Chromatography Analysis of N-Acyl Homoserine Lactone Hydrolysis by Paraoxonases / John F. Teiber and Dragomir I. Draganov -- Generation of Quorum Quenching Antibodies / Gunnar F. Kaufmann, Junguk Park, Alexander V. Mayorov, Diane M. Kubitz, and Kim D. Janda.