Total protein extraction with TCA-acetone / Valérie Méchin, Catherine Damerval, and Michel Zivy -- Phenol extraction of proteins for proteomic studies of recalcitrant plant tissues / Mireille Faurobert, Esther Pelpoir, and Jamila Chaïb -- Protein extraction from cereal seeds / Gérard Branlard and Emmanuelle Blancel -- Protein extraction from xylem and phloem sap / Julia Kehr and Martijn Rep -- Protein extraction from woody plants / Christophe Plomion and Céline Lalanne -- Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis / Klaas J. van Wijk, Jean-Benoit Peltier, and Lisa Giacomelli -- Isolation and subfractionation of plant mitochondria for proteomic analysis / Holger Eubel, Joshua L. Heazlewood, and A. Harvey Millar -- Extraction of nuclear proteins from root meristematic cells / Fernando González-Camacho and Francisco Javier Medina -- Isolation of nuclear proteins / Setsuko Komatsu -- Isolation of cell wall proteins from Medicago sativa stems / Bonnie S. Watson and Lloyd W. Sumner -- Plant plasma membrane protein extraction and solubilization for proteomic analysis / Véronique Santoni -- Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis / Thierry Rabilloud ... [et al.] -- Two-dimensional electrophoresis for plant proteomics / Walter Weiss and Angelika Görg -- Visible and fluorescent staining of two-dimensional gels / François Chevalier, Valérie Rofidal, and Michel Rossignol -- Two-dimensional differential in-gel electrophoresis (DIGE) of leaf and roots of Lycopersicon esculentum / Matthew Keeler ... [et al.] -- Quantitative analysis of 2D gels / Michel Zivy -- Multivariate data analysis of proteome data / Kåre Engkilde, Susanne Jacobsen, and Ib Søndergaard -- Edman sequencing of plant proteins from 2D gels / Setsuko Komatsu -- Peptide mass fingerprinting: identification of proteins by MALDI-TOF / Nicolas Sommerer, Delphine Centeno, and Michel Rossignol -- Protein identification using nano liquid chromatography-tandem mass spectrometry / Luc Negroni -- Two-dimensional nanoflow liquid chromatography-tandem mass spectrometry of proteins extracted from rice leaves and roots / Linda Breci and Paul A. Haynes -- Separation, identification, and profiling of membrane proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS / Wojciech Szponarski ... [et al.] -- The PROTICdb database for 2-DE proteomics / Olivier Langella, Michel Zivy, and Johann Joets -- Identification of phosphorylated proteins / Maria V. Turkina and Alexander V. Vener -- Plant proteomics and glycosylation / Anne-Catherine Fitchette ... [et al.] -- Blue-native gel electrophoresis for the characterization of protein complexes in plants / Jesco Heinemeyer, Dagmar Lewejohann, and Hans-Peter Braun -- Electroelution of intact proteins from SDS-PAGE gels and their subsequent MALDI-TOF MS analysis / Zhentian Lei ... [et al.] -- Generation of plant protein microarrays and investigation of antigen-antibody interactions / Birgit Kersten and Tanja Feilner -- Phosphorylation studies using plant protein microarrays / Tanja Feilner and Birgit Kersten.