عنوان

Protein purification protocols

(Number (ISBN

1588290670

(Number (ISBN

159259655X

(Number (ISBN

9781588290670

(Number (ISBN

9781592596553

Number

dltt

Title Proper

Protein purification protocols

General Material Designation

[Book]

Edition Statement

Second edition / edited by Paul Cutler

Specific Material Designation and Extent of Item

xii, 484 pages :

Other Physical Details

illustrations ;

Dimensions

26 cm

Series Title

Methods in molecular biology ;

Volume Designation

v. 244

Text of Note

Includes bibliographical references and index

Text of Note

General strategies / S. Doonan and P. Cutler -- Preparation of extracts from animal tissues / J.M. Skehel -- Protein extraction from plant tissues / R.J. Fido [and others] -- Extraction of recombinant protein from bacteria / A.F. McGettrick and D.M. Worrall -- Protein extraction from fungi / P.D. Bridge, T. Kokubun and M.S. Simmonds -- Subecllular fractionation of animal tissues / N.M. Ryan -- Subcellular fractionation of plant tissues: isolation of plastids and mitochondria / A.K. Tobin and C.G. Bowsher -- The extraction of enzymes from plant tissues rich in phenolic compounds / W.S. Pierpoint -- Avoidance of proteolysis in extracts / R.J. Beynon and S. Oliver -- Concentration of extracts / S. Doonan -- Making and changing buffers / S. Doonan -- Purification and concentration by ultrafiltration / P. Schratter -- Bulk purification by fractional precipitation / S. Doonan -- Ion-exchange chromatography / C. Selkirk -- Hydrophobic interaction chromatography / P.A. O'Farrell -- Affinity chromatography / P. Cutler -- Dye-ligand affinity chromatography / A.F. McGettrick and D.M. Worrall -- Lectin affinity chromatography / I. West and O. Goldring -- Immunoaffinity chromatography / P. Cutler -- Immobilized metal-ion affinity chromatography / T.T. Yip and T.W. Hutchens -- Chromatography on hydroxyapatite / S. Doonan -- Thiophilic affinity chromatography and related methods / P. Matejtschuk -- Affinity precipitation methods / J.A. Irwin and K.F. Tipton -- Isoelectric focusing / R. Westermeier -- Chromatofocusing / T.J. Mantle and P. Noone -- Size-exclusion chromatography / P. Cutler -- Fast protein liquid chromatography / D. Sheehan and S. O'Sullivan -- Reversed-phase chromatography of proteins / W.A. Neville -- Extraction of membrane proteins / K. Ohlendieck -- Removal of detergent from protein fractions / K. Ohlendieck -- Purification of membrane proteins / K. Ohlendieck -- Lyophilization of proteins / C. O'Fagain -- Storage of pure proteins / C. O'Fagain -- Electroelution of proteins from polyacrylamide gels / M.J. Dunn -- Electroblotting of proteins from polyacrylamide gels / M.J. Dunn -- Two-dimensional polyacrylamide gel electrophoresis for proteome analyses / N.A. Jones -- Microscale solution isoelectrofocusing: a sample prefractionation method for comprehensive proteome analysis / X. Zuo and D.W. Speicher -- Practical column chromatography / S. Doonan -- Detection methods / J. Mozdzanowski and S. Burman -- Peptide proteomics / D.E. McNulty and J.R. Slemmon -- Multidimensional liquid chromatography of proteins / R. Watson and T. Nadler -- Mass spectrometry / D.J. Bell -- Purification of therapeutic proteins / J. Bonnerjea -- Purification process scale-up / K. Prince and M. Smith

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Text of Note

In this new edition, the author completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. It brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and thie functions today

Proteins-- Purification, Laboratory manuals

Proteins-- isolation & purification, Laboratory Manuals

Proteins-- Isolation & purification, Laboratory manuals

Number

Number

Edition

Edition

Class number

Book number

Class number

Class number

Book Number

Book Number

Cutler, Paul,1962-

Date of Transaction

20160712072652.0

Electronic name

[Book]

Y