عنوان

Calcium-binding protein protocols /

(Number (ISBN

0585403236

(Number (ISBN

0585419728

(Number (ISBN

9780585403236

(Number (ISBN

9780585419725

Erroneous ISBN

089603688X

Erroneous ISBN

0896036898

Erroneous ISBN

1592591833

Erroneous ISBN

1592591841

Erroneous ISBN

9780896036888

Erroneous ISBN

9780896036895

Erroneous ISBN

9781592591831

Erroneous ISBN

9781592591848

Number

dltt

Title Proper

Calcium-binding protein protocols /

General Material Designation

[Book]

First Statement of Responsibility

edited by Hans J. Vogel.

Place of Publication, Distribution, etc.

Totowa, N.J. :

Name of Publisher, Distributor, etc.

Humana Press,

Date of Publication, Distribution, etc.

©2002.

Specific Material Designation and Extent of Item

1 online resource (2 volumes) :

Other Physical Details

illustrations (some color).

Series Title

Methods in molecular biology ;

Volume Designation

v. 172-173

Text of Note

880-01 v. 2. (v.173). Methods and techniques -- pt. 3. Methods and techniques to study calcium-binding proteins -- Quantitative analysis of Ca2+-binding by flow dialysis / Michio Yazawa -- Calcium binding to proteins studied via competition with chromophoric chelators / Sara Linse -- Deconvolution of calcium-binding curves: facts and fantasies / Jacques Haiech -- Absorption and circular dichroism spectroscopy / Stephen R. Martin -- Fourier transform infrared spectroscopy of calcium-binding proteins / Heinz Fabian -- Steady-state fluorescence spectroscopy / Aalim M. Weljie -- Fluorescence methods for measuring calcium affinity and calcium exchange with proteins / J. David Johnson -- Surface plasmon resonance of calcium-binding proteins / Karin Julenius -- Differential scanning calorimetry / Maria M. Lopez -- Isothermal titration calorimetry / Maria M. Lopez -- Multiangle laser light scattering and sedimentation equilibrium / Leslie D. Hicks -- Small-angle solution scattering reveals information on conformational dynamics in calcium-binding proteins and in their interactions with regulatory targets / Jill Trewhella -- Investigation of calcium-binding proteins using electrospray ionization mass spectrometry / Amanda L. Doherty-Kirby -- Synthetic calcium-binding peptides / Gary S. Shaw -- Proteolytic fragments of calcium-binding proteins / Richard D. Brokx -- Electron magnetic resonance studies of calcium-binding proteins / Lawrence J. Berliner -- Cadmium-113 and lead-207 NMR spectroscopic studies of calcium-binding proteins / Teresa E. Clarke -- Calcium-43 of NMR of calcium-binding proteins / Torbjörn Drakenberg -- Exploring familial relationships using multiple sequence alignment / Aalim M. Weljie -- Structure determination by NMR: isotope labeling / Monica X. Li -- Protein structure calculation from NMR data / Tapas K. Mal -- Shape and dynamics of a calcium-binding protein investigated by nitrogen-15 NMR relaxation / Jörn M. Werner -- The use of dipolar couplings for the structure refinement of a pair of calcium-binding EGF domains / Jonathan Boyd -- Vector geometry mapping: a method to characterize the conformation of helix-loop-helix calcium-binding proteins / Kyoko L. Yap -- Use of calmodulin anatgonists and S-100 protein interacting drugs for affinity chromatography / Ryoji Kobayashi -- Enzymatic assays to compare calmodulin isoforms, mutants, and chimeras / Michael P. Walsh -- Gene expression in transfected cells / Kate Hughes -- Monitoring the intracellular free Ca2+-calmodulin concentration with genetically-encoded fluorescent indicator proteins / Anthony Persechini -- Studying the spatial distribution of Ca2+-binding proteins: how does it work for calmodulin? / Katalin Török.

0

Text of Note

Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols-Volume 1: Reviews and Case Studies and Volume 2: Methods and Techniques-Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. This first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in dairy proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second companion volume, Methods and Techniques, focuses on cutting-edge experimental methods for studying solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding proteins in general. Comprehensive and highly practical, the two volumes of Calcium-Binding Protocols provide experimental and clinical biologists with a host of advanced experimental methods that can be applied successfully to the study of both existing and newly discovered members of this critically important class of proteins.

Title

Calcium-binding protein protocols

Calcium-binding proteins-- Research-- Methodology.

Calcium-Binding Proteins.

Research Design.

Class number

Book number

Vogel, Hans J.

Date of Transaction

20160621084321.0

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y