عنوان

Laboratory Methods in Enzymology :

(Number (ISBN

0124200370

(Number (ISBN

9780124200371

Number

dltt

Title Proper

Laboratory Methods in Enzymology :

General Material Designation

[Book]

Other Title Information

RNA /

First Statement of Responsibility

edited by Jon Lorsch

Specific Material Designation and Extent of Item

lvii, 413 pages :

Other Physical Details

illustrations ;

Dimensions

24 cm

Series Title

Methods in enzymology,

Volume Designation

v. 530

ISSN of Series

0076-6879 ;

Text of Note

Computational prediction of RNA secondary structure -- Reverse-transcription PCR (RT-PCR) -- Northern blotting -- Explanatory chapter: nuclease protection assays -- In vitro transcription from plasmid or PCR-amplified DNA -- Measurement of in vivo synthesis rates -- Method for measuring mRNA decay rate in saccharomyces cerevisiae -- Analysis of polysomes from bacteria -- Polysome profile analysis - yeast -- Polysome analysis of mammalian cells -- Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates -- Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3' ends, and poly(A) tails of RNA -- RNA radiolabeling -- Fluorescently labeling synthetic RNAs -- Analysis of RNA by analytical polyacrylamide gel -- RNA purification by preparative polyacrylamide gel -- Explanatory chapter: nucleic acid concentration determination -- RNA purification-precipitation methods -- Reverse transcriptase dideoxy sequencing of RNA -- RNA structure experimental analysis-chemical modification -- Structural analysis of RNA backbone using in-line probing

0

Biotechnology-- Methodology

Molecular biology-- Methodology

Class number

Book number

Lorsch, Jon

Date of Transaction

20131029014829.0

Cataloguing Rules (Descriptive Conventions))

rda

Electronic name

[Book]

Y