Part C,dollar5The contractile apparatus and the cytoskeleton /
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
Orlando :
Name of Publisher, Distributor, etc.
Academic Press,
Date of Publication, Distribution, etc.
1986
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
xxxi, 748 pages :
Other Physical Details
illustrations ;
Dimensions
24 cm
SERIES
Series Title
Methods in enzymology ;
Volume Designation
v. 134
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index
CONTENTS NOTE
Text of Note
Preparation and assay of cytoplasmic and secreted gelsolin / H.L. Yin -- Purification of severin / Susan S. Brown -- Isolation of fascin, and actin-bundling protein, and SU45, an actin-severing/capping protein from sea urchin eggs / seph Bryan -- Purification of the intestnal microvillus cytoskeletal proteins villin, fimbrin, and ezrin / Anthony Bretscher -- Purification of caldesmon / William Lynch, Anthony Bretscher -- Purification of fodrin from mammalian brain / Richard Cheney, Joel Levine, Mark Willard -- Purification of brain analogs of red blood cell membrane skeletal proteins: Ankyrin, protein 4.1 (synapsin), spectrin, and spectrin subunits / Vann Bennett, Anthony J. Baines, Jonathan Davis -- Purification and assay of vinculin, metavinculin, and talin / T. O'Halloran, L. Molonoy, K. Burridge -- Isolation of metavinculin from chicken smooth muscle / Janet D'Angelo Siliciano, Susan W. Craig -- Reversible assembly purification of microtubules without assemly-promoting agents and further purification of tubulijn, microtubule-associated proteins, and MAP fragments / Richard B. Vallee -- Purification of brain microtubules and microtubule-associated protein 1 using taxol / Richard B. Vallee -- Purification of microtubules and microtubule-associated proteins from sea urchin eggs and cultured mammalian cells using taxol, and use of exogenous taxol-stabilized brain microtubules for purifying microtubule-associated proteins / Richard B. Vallee, Christine A. Collins -- Isolation of sea urchin egg tubulin / H. William Detrich III -- Direct identification of microtubule-associated proteins be selective extraction of cultured cells / Frank Solomon -- Purification and assay of primate 210K microtubule-associated protein / Jeannette Chloë Bulinski -- Purification of tau protein from brain / David Drubin, Marc Kirschner -- Purification and assay of cold-stable microtubules and STOP protein / Robert L. Margolis, Charles T. Rauch, Didier Job -- Isolation and purification of tubulin tyrosine ligase / Jürgen Wehland, Heinz C. Schröder, Klaus Weber -- Proteolytic modification of tubulin / Luis Serrano, Francisco Wandosell, Javier de la Torre, Jesús Avila -- Isolation of sea urchin spindles and cytasters / Ryoko Kuriyama -- Isolation of native, membrane-containing mitotic apparatus from sea urchin embryos / Robert B. Silver -- Isolation of mitotic spindles from mammalian cells / R.C. Brady, M.J. Schibler, F. Cabral -- Isolation of mitotic spindles and spindle pole bodies from yeast / Jeremy S. Hyams, Stephen M. King -- Isolation of the erythrocyte marginal band / William D. Cohen, Mary F. Ginsburg -- Isolation of basal bodies from Chlamydomonas / William J. Snell -- Isolation of mammalian centrosomes / T.J. Mitchison, M.W. Kirschner -- Isolation of a kinetochore-centromere fraction from HeLa metaphase chromosomes / Manuel M. Valdivia, B.R. Brinkley -- Isolation of Chlamydomonas flagella and flagellar axonemes / George B. Witman -- Purification and characterization of Chlamydomonas flagellar dyneins / Stephen M. King, Tim Otter, George B. Witman -- Preparation and properties of dynein from Tetrahymena cilia / Kenneth A. Johnson -- Extraction and properties of dyneins from bull spermatozoa / Claude Gagnon -- Purification of cytoplasmic dynein from Strongylocentrotus sea urchin eggs / M.M. Pratt -- Purification of cytoplasmic dynein from sea urchin eggs / Shin-ichi Hisanaga, Hikoichi Sakai -- Separation of cytokeratin polypeptides by gel electrophoretic and chromatographic techniques and their identification by immunoblotting / Thomas Achtstaetter, Mechthild Hatzfeld, Roy A. Quinlan, David C. Parmelee, Werner W. Franke -- Isolation of intermediate filaments from neuronal tissues by cycles of assembly/disassembly / Robert V. Zackroff -- Purification of neurofilaments and their constituent polypeptides / Ronald K.H. Liem -- Isolation of paired helical filaments and amyloid fibers from human brain / Dennis J. Selkoe, Carmela R. Abraham -- Methods for studying spasmin / W. Brad Amos -- Isolation of the calcium-modulated contractile protein of striated flagellar roots / J.L. Salisbury, K.W. Aebig, D.E. Coling -- Isolation of nematode major sperm proteins / Samuel Ward, Michael R. Klass -- Antibody inhibitors of nonmuscle myosin function and assembly / Daniel P. Kiehart, Donald A. Kaiser, Thomas D. Pollard -- Preparation of antibodies reactive with specific regions of cytoskeletal proteins / Jeannette Chloë Bulinski, Gregg G. Gundersen -- Purification of isoform-selective actin antibody from polyclonal antiserum / Susan W. Craig, Sheila K. Sanders, JoséV. Pardo -- Analysis of cytoskeletal structures using blot-purified monospecific antibodies / J.B. Olmsted -- Preparation of N-ethylmaleimide-modified heavy meromyosin and its use as a functional probe of actomyosin-based motility / W. Zacheus Cande -- Erythro-9-[3-(2-hydroxynonyl)]adenine and Vanadate as probes for microtubule-based cytoskeletal mechanochemistry / Stephen M. Penningroth -- Preparation of a fluorescent analog: Acetamidofluoresceinyl-labeled Dictyostelium discoideum α-actinin / John R. Simon, D. Lansing Taylor -- Preparation, assay, and microinjection of fluorescently labeled cytoskeletal proteins: Actin, α-actinin, and vinculin / Thomas E. Kreis -- Preparation and characterization of fluorescent analogs of tubulin / Patricia Wadsworth, E.D. Salmon -- Myosin movement in Vitro: A quantitative assay using oriented actin cables from Nitella / Michael P. Sheetz, Steven M. Block, James A. Spudich -- Methods to study the microtubule-neurofilament network in Vitro / Eric J. Aamodt, Robley C. Williams Jr. -- Gel overlay methods for detecting specific protein-protein interactions / Joann J. Otto -- Viewing single microtubules by video light microscopy / Bruce J. Schnapp -- Agar overlay method: High-resolution immunofluorescence for the study of the contractile apparatus / Yoshio Fukui, Shigehiko Yumura, Toshiko K. Yumura, Hiroshi Mori -- Polyethylene glycol embedding protocol for immunofluorescence microscopy / John J. Wolosewick -- Preembedding for localization by electron microscopy of cytoskeletal antigens in cultured cell monolayers using gold-labeled antibodies / J. De Mey, G. Langanger, G. Geuens, R. Nuydens, M. De Brabander -- Quick freeze, deep etch of the cytoskeleton / Nobutaka Hirokawa -- Cryo-electron microscopy of unstained frozen-hydrated microtubules / Eva-Maria Mandelkow, Eckhard Mandelkow -- X-ray diffraction of cytoskeletal fibers / Eckhard Mandelkow -- Time-resolved x-ray scattering of microtubule assembly using synchrotron radiation / Eckhard Mandelkow, Joan Bordas -- Rapid kinetic analysis of mechanochemical adenosinetriphosphatases / Kenneth A. Johnson
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Title
Structural and contractile proteins. Part C, The contractile apparatus and the cytoskeleton.