عنوان

Modern techniques for pathogen detection /

(Number (ISBN

3527335161

(Number (ISBN

3527687971

(Number (ISBN

352768798X

(Number (ISBN

3527687998

(Number (ISBN

3527688005

(Number (ISBN

9783527335169

(Number (ISBN

9783527687978

(Number (ISBN

9783527687985

(Number (ISBN

9783527687992

(Number (ISBN

9783527688005

Erroneous ISBN

9783527335169

Number

dltt

Title Proper

Modern techniques for pathogen detection /

General Material Designation

[Book]

First Statement of Responsibility

edited by Jürgen Popp and Michael Bauer

Specific Material Designation and Extent of Item

1 online resource

Text of Note

Includes index

Text of Note

Includes bibliographical references at the end of each chapters and index

Text of Note

Unmet Medical Needs in Life-Threatening infections - Caring for the Critically Ill / Michael Bauer, Andreas Kortgen, Mervyn Singer -- Life Threatening Infections and Sepsis - Defining the Problem -- Sepsis as a "Hidden Healthcare Disaster" -- Microorganisms and Types of Infection Triggering Sepsis -- Emerging Problems Related to Resistance in Bacterial Infections -- The Role of Fungi and Viruses -- The Need for New Approaches in Diagnostics of Life-Threatening Infection and Sepsis -- Rapid and Sensitive Culture-Independent Strategies to Identify Blood Stream Infection -- Beyond Infection - Profiling the Immune Response of the Septic Host -- Host Factors Contributing to Pathogenesis of Sepsis -- References -- Identification Methods - An Overview / Oliwia Makarewicz, Claudia Stein, Wolfgang Pfister, Bettina Löffler, Mathias Pletz -- Taxonomy of Pathogenic Organisms -- Microscopic Methods -- Stains -- Autofluoresccnce -- Immunofluorescence -- In Situ Hybridization -- Dark-Field Microscopy -- Culture-Based Methods -- Blood Culture -- Automated Differentiation Systems -- API System -- Selective Agar -- Susceptibility Testing -- Other Methods -- Nucleic Acid-Based Techniques -- PCR -- Real-Time PCR -- Multiplex-PCR -- RAPD and rep-PCR -- Microaarrays -- RFLP -- Sequencing -- Ribosomal RNA Genes -- MLST -- NG Sequencing -- Serology -- Antigen-Antibody-Based Methods -- Agglutination -- Immunodiffusion -- Quantitative Immunoassays -- Automated Immunoassays -- Applications of Serological Test -- Types of Human Antibodies and What They Indicate? -- Validity of Antibodies against Opportunistic Species -- Specificity of Fungal Antigens -- Specific Urine Antigen Tests -- Biomarkers -- Conclusions and Perspectives -- References -- Nucleic Acid Amplification Techniques / Marc Lehmann, Roland P.H. Schmitz -- Introduction -- The Basic PCR Protocol -- Primer Design -- Specific versus Broad-Range Primers and the Fate of False Targeting -- Modified End-Point PCR Protocols -- Non-PCR NAT: isothermal Amplification Protocols -- Quantitative PCR -- Quantification in qPCR -- Melting Curve Analysis (MCA) -- Controls, Probing, and General Aspects of Result Interpretation -- Strategies in Amplicon Verification -- (DNA) Microarray -- Flow Cytometry -- Mass Spectrometry -- PCR Inhibitors -- Preanalyses -- Sample Volume -- Cell Lysis -- Nucleic Acid Isolation and Preparation -- Fields of PCR Application -- Sequencing -- Pyrosequencing -- Typing and Epidemiology -- Pathogen Detection in Complex Clinical Samples -- General Aspects of Result Interpretation -- Sensitivity and Specificity -- NAT versus Culture -- The Fate of Antibiotic Resistances Detection -- Microbial Trace Detection in BSI, Ascites, and Synovial Fluids -- Grown Blood Culture Media as Samples -- Upcoming Routine Solutions - Dawn of Assay Automation -- Conclusion and Perspective -- Acknowledgment -- References -- DNA Microarrays for Pathogen Detection / Holger Schulze, Maya Rubtsova, Till T. Bachmann -- Introduction -- DNA Microarrays for the Detection of Bacterial Pathogens -- Major Bacterial Diseases -- Blood Stream Infections/Sepsis -- Pneumonia -- Infectious Diarrhea -- Antibiotic Resistance Detection -- Gram-Negative Bacteria -- Gram-Positive Bacteria -- Staphylococcus aureus -- Mycobacteria -- DNA Microarrays for Virus Diagnostics -- Human Papillomaviruses -- Commercial HPV Tests -- Respiratory Viruses -- Enteric Viruses -- Hepatitis Viruses -- DNA Microarrays for Detection of Fungal Pathogens -- DNA Microarrays for Parasite Diagnostics -- Malaria -- Waterborne Parasites -- Other Parasites -- Conclusion an Outlook -- References -- MALDI-ToF / Stefan Zimmermann -- Introduction -- MALDI-ToF Technology -- Bacterial Identification Using Mass Spectrometry -- Culture-Independent Rapid Identification of Clinical Pathogens -- Antibiotic Susceptibility Testing Using Mass Spectrometry -- References -- IR and Raman Spectroscopy for Pathogen Detection / Ute Münchberg, Sandra Kloß, Dragana Kusic, Susann Meisel, Ralf Heinke, Stephan Stöckel, Petra Rösen, Jürgen Popp -- Introduction -- Bulk Analysis -- IR and NIR Absorption Spectroscopy -- Raman Spectroscopy with Excitation in the NIR Region -- Resonance Raman Spectroscopy -- Excitation with Visible Wavelengths -- UV-Resonance Raman Spectroscopy -- Surface-Enhanced Raman Spectroscopy -- The SERS Effect -- Analysis of Bulk Bacteria Samples with SERS -- Single-Cell Analyses -- Micro-Raman Spectroscopy with Excitation in the Visible or NIR Region -- Surface-Enhanced Raman Spectroscopy on Single Cells -- Conclusion and Outlook -- References -- FISH / Graeme N. Forrest, Jwan Mohammadi, Shahrzad Mohammadi -- Introduction -- FISH Techniques -- PNA FISH -- Original PNA FISH Platform -- PNA FISH Flow Cytometry -- Beacon-Based FISH (bbFISH) -- Clinical Implications of PNA FISH -- Gram-Positive Organisms -- Staphylococci -- Enterococci -- Gram-Negative Probes -- Candida -- Beta Hemolytic Streptococci -- Mycobacteria -- Helicobacter pylori -- Other Bacteria -- Parasites -- Summary and Outlook -- References -- Conclusions / Sibyll Pollok, Karina Weber, Michael Bauer, Jürgen Popp

0

International Standard Book Number

9783527687978

Pathogenic microorganisms-- Detection.

TEC-- 010000

Number

Edition

Class number

Bauer, Michael

Popp, Jürgen

Ohio Library and Information Network.

Date of Transaction

20160603081227.7

Cataloguing Rules (Descriptive Conventions))

pn

Electronic name

[Book]

Y