عنوان

Introduction to proteomics :

(Number (ISBN

0471754021

(Number (ISBN

9780471754022

Number

dltt

Title Proper

Introduction to proteomics :

General Material Designation

[Book]

Other Title Information

principles and applications /

First Statement of Responsibility

Nawin Mishra

Place of Publication, Distribution, etc.

Hoboken, N.J. :

Name of Publisher, Distributor, etc.

John Wiley & Sons,

Date of Publication, Distribution, etc.

c2010

Specific Material Designation and Extent of Item

xx, 180 p. :

Other Physical Details

ill. ;

Dimensions

24 cm

Series Title

Methods of biochemical analysis ;

ISSN of Series

146

Text of Note

Includes bibliographical references and index

Text of Note

(Publisher-supplied data) Table of Content 1. Chapter-1 Historical perspectives 1.1 Introduction to Proteomics 1.2 Proteome and Proteomics A. Proteins as cell?s way of accomplishing specific functions B. Pre-genomic proteomics 1.2.1 Genetics of Proteins A. One gene ?one enzyme theory B. One gene ?many proteins I. RNA Splicing II. RNA Editing References 5. Chapter-5 Proteomics of Protein?Protein interactions /Interactomes 5.1 In vivo analysis of Protein ?Protein Interactions A. Yeast two hybrids Assay B. Phage Display 5.2 In vitro Analysis of Protein Interactions A. Mass Spectrometry (MS) B. Functional Protein Micro array 5.3 In Silico analysis of Protein ?Protein Interactions ? Rosetta Stone and other computational analyses 5.4 Synthetic Genetic method to determine Protein-Protein Interactions 5.5 Interactomes 5.5.1 Prokaryotic interactomes 5.5.2 Eukaryotic interactomes A. Yeast B. Fly C. Worm D. Human E. Plasmodium F. Plants 5.5.3 Interactome during Human Development and Disease 5.6 Evolution and Conservation of Interactomes 5.7 Interactome and complexity of organism 5.8 Interaction of proteins with small molecules References III. RNA Silencing 1.2.2 Molecular Biology of genes and proteins 1.2.3 Protein Chemistry before proteomics References 2. Chapter-2 Proteomics - relation to Genomics, Bioinformatics 2.1 Genomics and Functional Genomics and their utilization in Proteomics 2.2 Bioinformatics and Computational aspects of Proteomics References

Text of Note

3 Methodology 3.1 Separation of proteins- via a multidimensional approach A. Electrophoresis I 2D Gel Electrophoresis II Capillary Electrophoresis B. Liquid Chromatography 3.2 Determination of the protein structure 3.2.1Determination of the Amino acid Sequences / primary structure of proteins I. Proteomics without Mass Spectrometry A. Amino acid sequence of a protein from DNA sequence B. Edman Degradation ? N-terminal amino acid sequence analysis3. Chapter- II. Proteomics based on Mass Spectrometry A. Mass Spectrometry ? Principle, Historical Background and Instrumentation B. Bottom-up and Top-down Mass Spectrometry C. Bioinformatics and Determination of Amino acid sequence of a protein 3.2.2 Analysis of Protein 3 Dimensional Structure A. X-ray Crystallography, B. Neutron Scattering C. NMR 3.3 Determination of the amount of proteins A. Quantitation of proteins in 2D gel ( Staining , densitometry) B. Differential gel analysis ( Dige) C. Quantitative Mass spectrometric protein ( Isotope labeling , ICAT) D. Protein Micro Array for large-scale analysis 3.4 Structural and Functional Proteomics References 4. Chapter ? 4 Proteomics of Protein Modifications 4.1. Phosphorylation and phosphoproteiomics 4.2. Glycoproteins and glycoproteiomics 4.3. Ubiquitination and ubiquitinomics 4.4. Miscellaneous modifications of proteins 6. Chapter -6 Applications I Application of Proteomics in Medicine: Proteomics, Human Disease and Medicine 6.1 Diseasome 6.2 Medical Proteomics: Proteomics of Human Body Parts A. Body fluid Proteome a. Blood /Plasma/Serum Proteome b. Salivary Proteome c. Others B. Liver Proteome C. Brain Proteome D. Heart/ cardiovascular Proteome E. Cancer Proteome F. Organelle Proteome G. Proteome of Human Parasites 6.3 Clinical Proteomics A. Genomics and Proteomics of human diseases B. Proteomics of Diagnostic markers and Drug Development C. Proteomics & Personalized Medicine D. Metabolomics 6.4 Meta Proteomics and Human Health II. Application of Proteomics in Biotechnology, Industry & others 6.5 Biotechnology & Industry of Drug Production 6.6. Metaproteomics of Microbial Fermentation a. Metabolite- Production b. Bioremediation c. Biomass and Energy production 6.7 Beef Industry 6.8. Bioterrorism & Bio-defense References 7. Chapter -7 Proteomics - Future 7.1 Technical Scope of Proteomics ? Beyond Protein Identification 7.2 Scientific scope of Proteomics- Control of Epigenesis 7.3 Medical Scope of Proteomics A. Human Diseases B. Development of Drugs C. Personalized Medicine D. Proteomics and Metabolomics 7.4 Proteomics, Energy Production and bioremediation 7.5 Proteomics and Biodefense

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Text of Note

"Proteomics provides an introductory insight on proteomics, discussing the basic principles of the field, how to apply specific technologies and instrumentation, and example applications in human health and diseases. With helpful study questions, this textbook presents an easy to grasp and solid overview and understanding of the principles, guidelines, and especially the complex instrumentation operations in proteomics for new students and research scientists. Written by a leader in proteomics studies, Proteomics offers an expert perspective on the field and the future of proteomics"--Provided by publisher

Proteomics, Textbooks

Proteome-- analysis

Proteomics

Number

Edition

Class number

Book number

Class number

Class number

Book Number

Book Number

Mishra, Nawin C., (Nawin Chandra),1935-

Date of Transaction

20100617113809.0

Electronic name

[Book]

Y