عنوان

Single-molecule enzymology :

(Number (ISBN

012809267X

(Number (ISBN

9780128092675

Number

dltt

Title Proper

Single-molecule enzymology :

General Material Designation

[Book]

Other Title Information

fluorescence-based and high throughput methods /

First Statement of Responsibility

edited by Maria Spies and Yann R. Chemla

Edition Statement

First edition

Specific Material Designation and Extent of Item

xviii, 598 pages :

Other Physical Details

illustrations (some color) ;

Dimensions

24 cm

Series Title

Methods in enzymology,

Volume Designation

volume 581

ISSN of Series

0076-6879 ;

Text of Note

Includes bibliographical references and indexes

Text of Note

Chapter 1. Direct fluorescent imaging of translocation and unwinding by individual DNA helicases -- Chapter 2. Single-molecule imaging with one color fluorescence -- Chapter 3. Measuring membrane protein dimerization equilibrium in lipid bilayers by single-molecule fluorescence microsopy -- Chapter 4. Fluorescent labeling of proteins in whole cell extracts for single-molecule imaging -- Chapter 5. Quantifying the assembly of multicomponent molecular machines by single-molecule total internal reflection fluorescence microscopy -- Chapter 6. How to measure separations and angles between intramolecular fluorescent markers -- Chapter 7. Precisely and accurately inferring single-molecule rate constants -- Chapter 8. Quantification of functional dynamics of membrane proteins reconstituted in nanodiscs membranes by single turnover functional readout -- Chapter 9. Putting Humpty-Dumpty together: clustering the functional dynamics of single biomolecular machines such as the splicosome -- Chapter 10. Single-molecule FRET to measure conformational dynamics of DNA mismatch repair proteins -- Chapter 11. Single-molecule confocal FRET microscopy to dissect conformational changes in the catalytic cycle of DNA topoisomerases -- Chapter 12. Probing the conformational landscape of DNA polymerases using diffusion-based single-molecule FRET -- Chapter 13. Methods for investigating DNA accessibility with single nucleosomes -- Chapter 14. Single-molecule fluorescence studies of fast protein folding -- Chapter 15. Single-molecule multicolor FRET assay for studying structural dynamics of biomolecules -- Chapter 16. A multicolor single-molecule FRET approach to study protein dynamics and interactions simultaneously -- Chapter 17. Interferometric scattering microscopy for the study of molecular motors -- Chapter 18. Enzyme kinetics in femtoliter arrays -- Author index -- Subject index

0

Enzyme kinetics

Fluorescence microscopy

Fluorescence spectroscopy, Congresses

Fluorescent probes, Congresses

Luminescent probes, Congresses

Number

Class number

Book number

Chemla, Yann R.

Spies, Maria

Date of Transaction

20170406100909.0

Cataloguing Rules (Descriptive Conventions))

rda

Electronic name

[Book]

Y