Physical methods for gene transfer / Mohammad Alsaggar and Dexi Liu -- Nonviral gene delivery systems by the combination of bubble liposomes and ultrasound / Daiki Omata, Yoichi Negishi, Ryo Suzuki, Yusuke Oda, Yoko Endo-Takahashi and Kazuo Maruyama -- Electroporation-mediated gene delivery / Jennifer L. Young and David A. Dean -- Hydrodynamic delivery / Takeshi Suda and Dexi Liu -- Sustained expression from DNA vectors / Suet Ping Wong, Orestis Argyros and Richard P. Harbottle -- Noncoding oligonucleotides : the belle of the ball in gene therapy / Ka-To Shum and John J. Rossi -- Self-amplifying mRNA vaccines / Luis A. Brito [and twelve others] -- Gene electrotransfer clinical trials / Richard Heller and Loree C. Heller