عنوان

The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations /

(Number (ISBN

0128011858

(Number (ISBN

9780128011850

Number

dltt

Title Proper

The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations /

General Material Designation

[Book]

First Statement of Responsibility

edited by Jennifer A. Doudna, Erik J. Sontheimer

Edition Statement

First edition

Specific Material Designation and Extent of Item

xx, 549 pages, 24 unnumbered pages of plates :

Other Physical Details

illustrations (some color) ;

Dimensions

24 cm

Series Title

Methods in enzymology,

Volume Designation

volume 546

ISSN of Series

0076-6879 ;

Text of Note

Includes bibliographical references and indexes

Text of Note

In vitro enzymology of Cas9 -- Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs -- Determining the specificities of TALENs, Cas9, and other genome-editing enzymes -- Genome engineering with custom recombinases -- Genome engineering in human cells -- Genome editing in human stem cells -- Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9 -- Genome editing using Cas9 nickases - Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases -- Adapting CRISPR/Cas9 for functional genomics screens -- The iCRISPR platform for rapid genome editing in human pluripotent stem cells -- Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired Nicks -- Genome editing for human gene therapy -- Generation of site-specific mutations in the rate genome via CRISPR/Cas9 -- CRISPR/Cas9-based genome editing in mice by single plasmid injection -- Imaging genomic elements in living cells using CRISPR/Cas9 -- Cas9-based genome editing in xenopus tropicalis -- Cas9-based genome editing in zebrafish -- Cas9-based genome editing in drosophila -- Transgene-free genome editing by germline injection of CRISPR/Cas RNA -- Cas9-based genome editing in arabidopsis and tobacco -- Mutliplex engineering of industrial yeast genomes using CRISPRm -- Protein engineering of Cas9 for enhanced function

0

Endonucleases, Laboratory manuals

Genetic engineering, Laboratory manuals

Genomics, Laboratory manuals

Restriction enzymes, DNA, Laboratory manuals

Number

Edition

Class number

Book number

Doudna, Jennifer A.

Sontheimer, Erik J.

Date of Transaction

20160712084354.0

Cataloguing Rules (Descriptive Conventions))

rda

Electronic name

[Book]

Y