عنوان

Riboswitch discovery, structure and function

(Number (ISBN

012801122X

(Number (ISBN

9780128011225

Number

dltt

Title Proper

Riboswitch discovery, structure and function

General Material Designation

[Book]

Specific Material Designation and Extent of Item

xxiii, 546 pages :

Other Physical Details

illustrations, 21 leaves of plates ;

Dimensions

24 cm

Series Title

Methods in enzymology,

Volume Designation

549

ISSN of Series

0076-6879 ;

Text of Note

Includes bibliographical references and indexes

Text of Note

Part I: Riboswitch Discovery: Riboswitch discovery by combining RNA-seq and genome-wide identification of transcriptional start sites -- Discovering human RNA aptamers by structure-based bioinformatics and genome-based in vitro selection -- Part II: Synthesis and Sample Prep Methods for Large RNAs: Affinity purification of invitro transcribed RNA with homogeneous ends using a 3'-ARiBo tag -- Deoxyribozyme-mediated ligation for incorporating EPR spin labels and reporter groups into RNA -- A flexible, scalable method for preparation of homogeneous aminoacylated tRNAs -- Synthesis of a biotinylated photocleavable nucleotide monophosphate for the preparation of natively folded RNAs -- Chemo-enzymatic synthesis of selectively 13C/15N-labeled RNA for NMR structural and dynamics studies -- Part III: Structure and Folding: SHAPE analysis of small RNAs and riboswitches -- Experimental approaches for measuring pKa's in RNA and DNA -- Crystallographic analysis of TPP riboswitch binding by small-molecule ligands discovered through fragment-based drug discovery approaches -- Methods for using new conceptual tools and parameters to assess RNA structure by small-angle X-ray scattering -- Part IV: Dynamics: Use of 19F NMR methods to probe conformational heterogeneity and dynamics of exchange in functional RNA molecules -- Site-directed spin-labeling strategies and electron paramagnetic resonance spectroscopy for large riboswitches -- Using sm-FRET and denaturants to reveal folding landscapes -- Riboswitch structure and dynamics by smFRET microscopy -- Ribosome structure and dynamics by smFRET microscopy -- Unraveling the thermodynamics and kinetics of RNA assembly: surface plasmon resonance, isothermal titration calorimetry, and circular dichroism -- Part V: Ligand Interactions: ITC analysis of ligand binding to PreQ1 riboswitches -- Facile characterization of aptamer kinetic and equilibrium binding properties using surfce plasmon resonance -- The AdoCbl-riboswitch interaction investigated by in-line probing and surface plasmon resonance spectroscopy (SPR) -- Assessing RNA interactions with proteins by DRaCALA

Text of Note

Riboswitch discovery by combining RNA-seq and genome-wide indentification of transcriptional start sites -- Discoverying human RNA aptamers by structure-based bioinformatics and genome-based in vitro selection -- Affinity purification of in vitro transcribed RNA with homogenous ends using a 3'-ARiBo tag -- Deoxyribozyme-mediated ligation for incorporating EPR spin labels and reporter groups into RNA -- A flexible, scalable method for preparation of homogenous aminoacylated tRNAs -- Synthesis of a biotinylated photocleavable nucleotide monophosphate for the preparation of natively folded RNAs -- Chemo-enzymatic synthesis of selectively 13C/15N-labeled RNA for NMR structural and dynamics studies -- SHAPE analysis of small RNAs and riboswitches -- Experimental approaches for measuring pKa's in RNA and DNA -- Crystallographic analysis of TPP riboswitch binding by small-molecule ligands discovered through fragment-based drug discovery approaches -- Methods for using new conceptual tools and parameters to assess RNA structure by small-angle X-ray scattering -- Use of 19F NMR methods to probe conformational heterogeneity and dynamics of exchange in functional RNA molecules -- Site-directed spin-labeling strategies and electron paramagetic resonance spectroscopy for large riboswitches -- Using sm-FRET and denaturants to reveal folding landscapes -- Riboswitch structure and dynamics by smFRET microscopy -- Ribosome structure and dynamics by smFRET microscopy -- Unraveling the thermodynamics and kinetics of RNA assembly: surface plasmon resonance, isothermal titration calorimetry, and circular dichroism -- ITC analysis of ligand binding to PreQ1 riboswitches -- Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance -- The AdoCbl-riboswitch interaction investitaged by in-line probing and surface plasmon resonance spectroscopoy (SPR) -- Assessing RNA interactions with proteins by DRaCALA

0

0

Receptor-ligand complexes

Riboswitches

Small interfering RNA

Alvarado, Luigi J.

Burke, Donald H.

Date of Transaction

20150213100638.0

Cataloguing Rules (Descriptive Conventions))

rda

Electronic name

[Book]

Y