عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
INTERNATIONAL STANDARD BOOK NUMBER
(Number (ISBN
1603273689
(Number (ISBN
9781603273688
Erroneous ISBN
1603273697
Erroneous ISBN
9781603273695
NATIONAL BIBLIOGRAPHY NUMBER
Number
dltt
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
Lipoproteins and cardiovascular disease
General Material Designation
[Book]
Other Title Information
methods and protocols /
First Statement of Responsibility
edited by Lita A. Freeman, Cardiovascular & Pulmanary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health (NIH), Bethesda, MD, USA.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
New York :
Name of Publisher, Distributor, etc.
Springer [distributor],
Date of Publication, Distribution, etc.
c2013.
SERIES
Series Title
Methods in molecular biology,
Series Title
Springer protocols
Volume Designation
1027
ISSN of Series
1064-3745 ;
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index.
CONTENTS NOTE
Text of Note
Cloning Full-Length Transcripts and Transcript Variants Using 5 and 3 RACE -- Monitoring Gene Expression: Quantitative Real-Time RT-PCR -- Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases -- Northern Analysis of Gene Expression -- Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions -- Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research -- Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research -- Strategies for Designing Transgenic DNA Constructs -- Purification of Plasmid and BAC Transgenic DNA Constructs -- Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production -- Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes -- Generation of General and Tissue-Specific Gene Knockout Mouse Models -- Adeno-Associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism -- Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology -- Chromatin Immunoprecipitation -- Measurement of Lecithin:Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate -- Native-Native 2D Gel Electrophoresis for HDL Subpopulation Analysis -- Western Blots.
0
SUMMARY OR ABSTRACT
Text of Note
The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies. In Lipoproteins and Cardiovascular Disease: Methods and Protocols, experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used. Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Lipoproteins and Cardiovascular Disease: Methods and Protocols serves both novices and experts alike as a complete guide for any researcher with an interest in lipoproteins and their significant biological effects.--
TOPICAL NAME USED AS SUBJECT
Cardiovascular system-- Diseases-- Genetic aspects.
Lipoproteins-- Health aspects.
Cardiovascular Diseases-- genetics.
Gene Expression Profiling.
Genotyping Techniques.
Lipoproteins-- pharmacokinetics.
PERSONAL NAME - ALTERNATIVE RESPONSIBILITY
Freeman, Lita A.,1958-
ORIGINATING SOURCE
Date of Transaction
20181223155434.0
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب [Book]
Y
