عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
INTERNATIONAL STANDARD BOOK NUMBER
(Number (ISBN
1493925954
(Number (ISBN
9781493925957
Erroneous ISBN
1493925962
Erroneous ISBN
9781493925964
NATIONAL BIBLIOGRAPHY NUMBER
Number
dltt
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
DNA replication
General Material Designation
[Book]
Other Title Information
methods and protocols /
First Statement of Responsibility
edited by Sonya Vengrova, Coventry, UK, Jacob Dalgaard, Warwick Medical School, University of Warwick, Coventry, UK.
EDITION STATEMENT
Edition Statement
Second edition.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
New York :
Name of Publisher, Distributor, etc.
Humana Press,
Date of Publication, Distribution, etc.
[2015]
SERIES
Series Title
Methods in molecular biology,
Series Title
Springer protocols
Volume Designation
1300
ISSN of Series
1064-3745 ;
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index.
CONTENTS NOTE
Text of Note
A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analyzing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast -- EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection and Sequencing of Okazaki Fragments In S. cerevisiae -- ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin -- Chromatin Immunoprecipitation to Detect DNA Replication and Repair Factors -- Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism -- Single-Molecule Observation of Prokaryotic DNA Replication -- Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.
0
SUMMARY OR ABSTRACT
Text of Note
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.
UNIFORM TITLE
General Material Designation
DNA replication (Vengrova)
TOPICAL NAME USED AS SUBJECT
DNA replication-- Research-- Methodology.
DNA replication, Laboratory manuals.
DNA Replication.
PERSONAL NAME - ALTERNATIVE RESPONSIBILITY
Dalgaard, Jacob Z.
Vengrova, Sonya.
ORIGINATING SOURCE
Date of Transaction
20181223150723.0
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب [Book]
Y
