edited by Martine Dieuaide-Noubhani, Ana Paula Alonso
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
xi, 365 pages :
Other Physical Details
illustrations (some color) ;
Dimensions
26 cm
SERIES
Series Title
Methods in molecular biology,
Series Title
Springer protocols
Volume Designation
1090
ISSN of Series
1064-3745 ;
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index
CONTENTS NOTE
Text of Note
Application of Metabolic Flux Analysis to Plants -- Metabolic Network Reconstruction and Their Topological Analysis -- 14C pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism -- Optimization of Steady-state 13C-labeling Experiments for Metabolic Flux Analysis -- Quantification of 13C Enrichments and Isotopomer Abundances for Metabolic Flux analysis using 1D NMR Spectroscopy -- Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR -- Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS -- Quantifying 13C-labeling in Free Sugars and Starch by GC-MS -- Liquid Chromatography Tandem Mass Spectrometry for Measuring 13C-labeling in Intermediates of the Glycolysis and Pentose-phosphate Pathway -- In Vivo NMR for 13C metabolic? Flux Analysis -- Steady State and Instationary Modeling of Proteinogenic and Free Amino Acid Isotopomers for Flux Quantification -- Isotopically Nonstationary MFA (INST-MFA) of Autotrophic Metabolism -- Simulating Labeling to Estimate Kinetic Parameters for Flux Control Analysis -- High-throughput Data Pipelines for Metabolic Flux Analysis in Plants -- Analysis of Enzyme Activities -- Analytical Kinetic Modeling: A Practical Procedure -- Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling -- Flux Variability Analysis: Application to Developing Oilseed Rape Embryos using Toolboxes for Constraint-Based Modeling -- Plant Genome-Scale Modeling and Implementation -- 34S and 15N Labelling to Model S and N Flux in Plants and Determine the Different Components of N and S use Efficiency -- Ecophysiological Process-based Model to Simulate Carbon Fluxes in Plants