عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
INTERNATIONAL STANDARD BOOK NUMBER
(Number (ISBN
0896035735 (acid-free paper)
(Number (ISBN
9780896035737 (acid-free paper)
NATIONAL BIBLIOGRAPHY NUMBER
Number
b429155
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
Transcription factor protocols
General Material Designation
[Book]
First Statement of Responsibility
edited by Martin J. Tymms.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
Totowa, N.J. :
Name of Publisher, Distributor, etc.
Humana Press,
Date of Publication, Distribution, etc.
c2000.
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
x, 306 p. :
Other Physical Details
ill. ;
Dimensions
23 cm.
SERIES
Series Title
Methods in molecular biology ;
Volume Designation
v. 130
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index.
CONTENTS NOTE
Text of Note
Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis.
0
SYSTEM REQUIREMENTS NOTE (ELECTRONIC RESOURCES)
Text of Note
Mode of access: World Wide Web.
OTHER EDITION IN ANOTHER MEDIUM
Title
Transcription factor protocols
PIECE
Title
OhioLINK electronic book center
Title
SpringerLink
TOPICAL NAME USED AS SUBJECT
Transcription factors, Laboratory manuals.
PERSONAL NAME - ALTERNATIVE RESPONSIBILITY
Tymms, Martin J.
ORIGINATING SOURCE
Date of Transaction
20081117041421.0
ELECTRONIC LOCATION AND ACCESS
Electronic name
مطالعه متن کتاب [Book]
Y
