edited by Paul D. Fey, Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, NE, USA.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
New York :
Name of Publisher, Distributor, etc.
Humana Press,
Date of Publication, Distribution, etc.
[2014]
SERIES
Series Title
Methods in molecular biology,
Series Title
Springer protocols,
Volume Designation
1106
ISSN of Series
1064-3745 ;
ISSN of Series
1949-2448
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index.
CONTENTS NOTE
Text of Note
Clinical characteristics of infections in humans due to Staphylococcus epidermidis / Mark E. Rupp -- Staphylococcus epidermidis pathogenesis / Michael Otto -- Identification of Staphylococcus epidermidis in the clinical microbiology laboratory by molecular methods / Amity L. Roberts -- Pulsed field gel electrophoresis of Staphylococcus epidermidis / Richard V. Goering and Paul D. Fey -- Multilocus sequence typing of Staphylococcus epidermidis / Jonathan C. Thomas and D. Ashley Robinson -- Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis / Greg A. Somerville and Robert Powers -- The isolation and analysis of phenol-soluble modulins of Staphylococcus epidermidis / Hwang-Soo Joo and Michael Otto -- Genetic manipulation of staphylococci / Jeffrey L. Bose -- Isolation of chromosomal and plasmid DNA from Staphylococcus epidermidis / Jill K. Lindgren -- Isolation of Staphylococus sp. RNA / Tess Eidem -- Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis / Katherine L. Maliszewski and Austin S. Nuxoll -- Methods to generate a sequence-defined transposon mutant library in Staphylococcus epidermidis strain 1457 / Todd J. Widhelm [and four others] -- Examination of Staphylococcus epidermidis biofilms using flow-cell technology / Derek E. Moormeier and Kenneth W. Bayles -- Rapid quantitative and qualitative analysis of biofilm production by Staphylococcus epidermidis under static growth conditions / Elaine M. Waters [and five others] -- Bacteriophage transduction in Staphylococcus epidermidis / Michael E. Olson and Alexander R. Horswill -- Mouse model of post-arthroplasty Staphylococcus epidermidis joint infection / Tyler D. Scherr [and five others] -- A mouse model of Staphylococcus catheter-associated biofilm infection / Cortney E. Heim, Mark L. Hanke, and Tammy Kielian -- Generation of a central nervous system catheter-associated infection in mice with Staphylococcus epidermidis / Jessica N. Snowden -- Rat jugular catheter model of biofilm-mediated infection / Carolyn R. Schaeffer, Keith M. Woods, and G. Matthew Longo.
0
SUMMARY OR ABSTRACT
Text of Note
Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. --